FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_abdna_daplib_nebset1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_abdna_daplib_nebset1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630351
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG11901.2732926E-117.647058570
AGCGTCG12706.002665E-117.165354770
GCGTCGT11755.9299055E-107.14893669
AGAGCGT16050.06.978193369
GAGCGTC13553.6379788E-116.974169770
CCCATGT6050.0049715126.363636559
CGTGTAG8050.00257179285.65217468
CAGATCG12058.237797E-55.22821664
TCGGAAG25703.6379788E-125.175097570
GTCGTGT10350.00167803435.07246470
Produced by FastQC (version 0.11.5)