FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_abdna_ablib_nebset1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_abdna_ablib_nebset1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736489
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCC5100.00682855356.862745318
CTTTACG8854.193318E-66.72316361
CTATGAA26950.05.58441541
CTATTAA27800.05.53956841
TATGACT62800.05.5175162
GCAACCG14654.0195846E-75.494880716
ATATATG51700.05.34816271
TGTCATG48400.05.278925412
CGTGTAG34350.05.196506570
CTATAAA29050.05.18072271
GATGTCA41900.05.178997510
CTCCTCT21201.9135769E-95.1179241
GTCATGT47300.05.10570813
ATATGAC59900.05.08347231
ATGACTT64200.05.07009363
CCCGATC22101.0732037E-95.067873551
ATTATCG11753.1654755E-45.063831
CTATACA15902.041359E-65.0628931
CTTAGAG15254.97164E-65.049181
CTATAGC9750.00440291555.0256411
Produced by FastQC (version 0.11.5)