FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_abdna_ablib_dapv2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_abdna_ablib_dapv2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4680342
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG5153.6379788E-1212.912621570
GGTCGCC3853.2775552E-610.90909155
TGGTCGC5201.1840766E-69.42307854
GCCGTAT6201.2695462E-57.90322659
ACGCCTA4650.0027782397.526881748
GCTCCCC4700.0030848197.44680949
AGCGTGC7151.0596119E-57.34265770
TGTAGGC30550.07.21767670
AGTGTAG52500.07.13333370
AGGCTAT27700.06.949458670
TCGGTGG10754.0952727E-86.837208750
CGCCGTA5200.0082392176.730769658
GTAGGCT28250.06.690264768
AGAGTGT60400.06.605960470
GTGTAGG162900.06.553170
CGTGTAG144700.06.458189570
TAGGCTA29250.06.3418869
CTATAAG18250.06.3287671
CTCGGTG10651.7347193E-66.24413249
GGTGGTC11852.5907684E-76.202531352
Produced by FastQC (version 0.11.5)