FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n02_abdna_ablib_dapv2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n02_abdna_ablib_dapv2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4680342
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG4901.8189894E-1213.57142970
GGTCGCC4056.0057337E-610.3703755
CGGGCAG3200.0088806598.7564
ACGCCTA4602.7116796E-48.36956648
TGGTCGC5552.7309725E-58.19819854
TGTAGGC31150.07.977527670
GCCGTAT6552.6362211E-57.480916559
CGCCGTA4900.00463213637.14285758
TCGGTGG10552.8603608E-86.96682550
AGTGTAG52750.06.96682570
AGGCTAT28150.06.962770
AGAGTGT61550.06.880584770
GTAGGCT28500.06.877193568
CTCGGTG9854.516569E-76.75126949
GCTATAG24400.06.74180369
GGTGGTC11451.9163053E-86.724890752
GTGTAGG164350.06.580468770
GGGAAAG94400.06.562569
CTATAAG16955.456968E-126.40118031
CGTGTAG144950.06.398758470
Produced by FastQC (version 0.11.5)