FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048691
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320443.055618861990806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGT1150.001007937618.26086868
GCGGTCG1450.004739535514.482758570
ACGTCTG5201.1405669E-48.07692366
ACACGTC5804.7242716E-57.844827769
GAGATCG4600.00249501487.608695570
CAGATCG5159.0896833E-47.47572869
CGTCTGA4750.00341563587.368420667
GCACACG7006.3188185E-57.067
CACACGT6602.3340655E-46.893939568
CACGTCT6107.1712356E-46.88524670
CGGAAGA14601.5061232E-96.23287769
TCGGAAG14852.224624E-96.127946468
AGCACAC8255.332762E-45.93939469
TAGATCG9002.5619642E-45.83333370
ATCGGAA16801.020453E-95.83333370
GATCGGA17653.3815013E-95.552407769
AGAGCAC9700.00412053665.051546667
Produced by FastQC (version 0.11.5)