FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_mldna_neblib_nebset1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_mldna_neblib_nebset1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4059822
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4500.017.8888946
GTATGCC6300.013.33333345
CGTATGC5800.012.67241444
TGCCGTC5601.8189894E-1111.87548
ATGCCGT6401.8189894E-1110.937547
GCCGTCT5552.2937456E-910.7207249
ACTGACC8807.488779E-97.95454532
CACTGAC9552.6378984E-76.96335131
CTCGTAT8158.947907E-66.871165342
TCGTATG10251.243825E-76.82926943
ACGTCTG11158.204552E-86.591928515
GTCACTG11551.5983278E-76.363636529
CGTCTGA12953.3089236E-86.21621616
GCACACG11601.1599222E-66.034482511
CCGTCTT11355.128524E-65.85903150
ACACGTC13901.3969839E-75.79136713
CACACGT14101.8639912E-75.709220467
CCAGTCA11503.9343286E-55.47826126
CACCGGT8500.00489347755.35294124
CAGTCAC11151.447329E-45.33632327
Produced by FastQC (version 0.11.5)