FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_mldna_neblib_nebset1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_mldna_neblib_nebset1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4059822
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4450.018.87640446
CGTATGC5950.012.94117644
GTATGCC6350.012.67716645
TGCCGTC5104.3655746E-1112.352941548
ATGCCGT6100.012.04918147
GCCGTCT5703.0377123E-1011.05263149
CGACTGG3550.0020759618.873239555
ACTGACC9051.4715624E-98.12154732
CACTGAC9453.4197E-97.777777731
CTCGTAT7856.3386324E-77.579617542
TCGTATG10351.981607E-87.101449543
ACGTCTG11151.1286829E-86.9058315
GTCACTG11201.2341843E-86.87529
GCACACG11702.0383231E-76.28205111
CGTCTGA13204.8910806E-86.09848516
CTGACCA12406.053233E-75.92741933
CCGTCTT11355.128524E-65.85903150
ACACGTC14505.379661E-85.793103713
CCAGTCA11657.969615E-65.708154726
TCCAGTC12051.4053945E-55.518672525
Produced by FastQC (version 0.11.5)