FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_mldna_neblib_dapv2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_mldna_neblib_dapv2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250834
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG3900.036.79487233
TATGCCG3850.035.45454448
TGCCGTC3950.034.55696550
CGTATGC4650.033.1182846
ATGCCGT4450.030.67415649
GCCGTCT4600.030.43478451
GTATGCC5000.028.747
TCCGCGA5400.025.92592634
CCGCGAA5300.025.0943435
CGCGAAA5550.023.33333436
CACTCCG6450.023.33333231
TCGTATG6450.022.2480645
CTCGTAT6000.022.16666844
ACTCCGC8300.017.71084232
CCGTCTT8350.016.76646652
GCGAAAT8000.016.187537
TCTCGTA8450.015.73964543
CAGTCAC10900.014.44954227
GTCACTC10500.014.33333329
CTGCTTG9900.013.08080959
Produced by FastQC (version 0.11.5)