FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_mldna_neblib_dapv2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_mldna_neblib_dapv2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250834
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG3650.035.4794533
TATGCCG3550.034.50704248
TGCCGTC3800.032.23684350
CGTATGC4600.030.43478446
ATGCCGT4100.029.8780549
GCCGTCT4550.027.69230751
GTATGCC4850.026.7010347
TCCGCGA5000.025.234
CCGCGAA5050.023.56435635
CGCGAAA5150.022.42718336
CTCGTAT5650.022.30088444
CACTCCG6200.022.01612931
TCGTATG6250.020.72000145
ACTCCGC8150.016.74846632
TCTCGTA8100.015.55555543
GCGAAAT8000.014.87499937
CCGTCTT8550.014.73684152
GTCACTC10250.013.31707329
CAGTCAC11100.012.92792927
CTGCTTG9800.012.14285759
Produced by FastQC (version 0.11.5)