FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_mldna_daplib_nebset1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_mldna_daplib_nebset1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736489
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTC1450.004742551614.482758515
GCAGCGT3300.00106667779.54545536
CACGCCG3050.00598914879.18032730
CTTAGGG6208.646786E-46.77419331
CTATCTG10205.713204E-66.17647081
TATAGGC8154.572323E-46.01226952
ATATAGG19253.6379788E-126.01
CTTTAAG35300.05.6515581
CTATAAG13706.4365486E-75.62043761
ATATAAG33700.05.5044511
CTTACGA7650.00907973855.4901961
CTTAAGG11051.2639585E-45.38461541
GTCGAAT58050.05.366063666
GGAGTCG45900.05.33769163
CAGTCAC17155.837319E-85.306122367
ATATGAC71250.05.256141
CGAATAT60350.05.219552568
TCGAATA61700.05.218800567
AGTCACC16104.7604226E-75.21739168
CGTCTGA33900.05.162241569
Produced by FastQC (version 0.11.5)