FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_abdna_neblib_nebset1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_abdna_neblib_nebset1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558946
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4000.031.50000246
GCCGTCT3850.030.9090949
TGCCGTC3750.030.80000148
ATGCCGT4450.028.31460647
GTATGCC4950.025.45454645
CGTATGC5350.024.20560844
CGATGTA6100.020.65573934
CTCGTAT6800.020.0735342
GTCACCG6650.020.029
CACCGAT6400.019.687531
CCGATGT6400.018.5937533
ACCGATG6800.017.532
TCACCGA8050.017.39130430
TCGTATG7700.017.27272643
TCTCGTA7350.016.66666641
CCGTCTT7450.016.44295550
CAGTCAC8500.016.05882327
AGTCACC8700.014.885056528
CCAGTCA8900.014.15730326
CTGCTTG9450.013.70370457
Produced by FastQC (version 0.11.5)