FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_abdna_neblib_nebset1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_abdna_neblib_nebset1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558946
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3900.033.20512846
GCCGTCT3750.032.66666849
TGCCGTC3800.031.31578848
ATGCCGT4500.028.77777547
GTATGCC4950.026.16161745
CGTATGC5350.024.85981444
CGATGTA6300.021.1111134
GTCACCG6650.020.52631629
CTCGTAT6850.020.43795642
CACCGAT6300.020.031
CCGATGT6350.019.2913433
TCGTATG7500.018.243
ACCGATG6950.017.62589832
TCACCGA8250.017.3939430
CAGTCAC8250.016.96969827
CCGTCTT7550.016.68874250
TCTCGTA7700.016.36363641
AGTCACC8650.015.37572228
CCAGTCA8500.015.23529426
CTGCTTG9550.013.56020957
Produced by FastQC (version 0.11.5)