FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_abdna_neblib_dapv2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_abdna_neblib_dapv2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994192
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT10250.10309879781772535TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2500.042.049
TATGCCG2600.040.38461748
TGCCGTC2600.039.03846450
GCCGTCT2900.037.4137951
CGTATGC3100.033.87096846
CCGGCTA3200.032.812533
ACCGGCT3500.032.032
CACCGGC3400.031.91176431
CTCGTAT3300.031.81818444
GTATGCC3250.031.23076847
TCGTATG3450.030.43478245
CGGCTAT3450.030.43478234
GGCTATG4250.024.70588135
CTGCTTG4450.023.59550559
CCGTCTT4350.023.33333452
TCTCGTA4000.022.75000243
AGTCACC4750.022.10526528
GTCACCG5000.021.029
CAGTCAC5200.020.86538527
CCAGTCA5400.020.7407426
Produced by FastQC (version 0.11.5)