FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_abdna_neblib_dapv2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_abdna_neblib_dapv2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994192
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT10250.10309879781772535TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2650.040.94339849
TATGCCG2700.040.18518448
TGCCGTC2650.039.62264350
GCCGTCT3000.037.33333251
CGTATGC3100.035.046
CCGGCTA3250.033.38461333
CTCGTAT3300.032.87878844
ACCGGCT3550.032.53521332
CACCGGC3500.032.031
GTATGCC3300.031.81818447
TCGTATG3500.031.045
CGGCTAT3550.030.56338134
GGCTATG4300.025.2325635
CTGCTTG4500.024.1111159
CCGTCTT4400.023.86363652
TCTCGTA4100.023.0487843
AGTCACC4750.022.84210628
CAGTCAC5050.022.17821727
GTCACCG5000.021.69999929
CCAGTCA5450.021.19266126
Produced by FastQC (version 0.11.5)