FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_abdna_daplib_dapv2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_abdna_daplib_dapv2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727335
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAGTC16000.010.570
CTCCAGT18250.09.58904169
ACTCCAG20850.09.06474870
CAGTCAC11800.08.89830569
CCAGTCA14550.08.65979470
AGTCACT11750.08.340425570
CGTCTGA45750.08.1092970
GTCACTC8252.0037987E-88.06060769
CACGTCT58200.07.998282470
TCTGAAC38250.07.86928170
CGGTGTC4100.00760391457.6829276
GAACTCC28300.07.667844370
ACGTCTG49600.07.550403669
ACACGTC63100.07.377179669
CACACGT70250.07.37366670
TCTCGCG4900.00462859647.14285769
TGAACTC33600.07.08333370
CTGAACT33850.07.03101970
GTCTGAA43750.06.9669
GCACACG75850.06.829268569
Produced by FastQC (version 0.11.5)