FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_abdna_ablib_nebset1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_abdna_ablib_nebset1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736489
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCT3950.00544710977.97468362
TATAGGC7803.583831E-56.7307692
ATAGGCC5850.00350010286.5811973
CTATCTG9751.8824276E-56.1025641
CTTACGA7200.00464568335.8333331
ATATAGG19453.274181E-115.75835471
CTAAGTG9354.3099097E-45.61497351
CTATAAG13756.9046655E-75.60000041
ATATAAG33150.05.5957771
CTTTAAG35700.05.4901961
CTATTAG15452.0284097E-75.43689351
CTTAATG17256.645314E-85.2753621
CAACCGC9300.00244147915.26881748
CTATTCG9300.00244147915.2688171
ATTAGAG22657.2759576E-115.25386331
AGTCACC16155.0744893E-75.201238669
ATCCTGT10100.00121328295.198021
CTATGAA26301.8189894E-125.1901141
CTATGTT21656.166374E-105.17320971
CTTAAGG10906.0110004E-45.13761471
Produced by FastQC (version 0.11.5)