FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_abdna_ablib_dapv2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_abdna_ablib_dapv2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4680342
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT5551.4551915E-1111.98198151
TATGCCG6908.281859E-99.13043448
TGCCGTC7251.929402E-88.68965550
ATGCCGT9101.6407284E-98.07692349
GTATGCC9151.2493183E-77.2677647
CCAGTCA54250.07.225806770
CAGTCAC46750.07.03743369
TCCAGTC58150.06.801375469
CTCCAGT66900.06.80119670
GTCGGCA6208.6481415E-46.774193345
CGTATGC10552.1022242E-76.635071346
ACTCCAG71850.06.47877569
CTATACG7601.855424E-46.44736861
TGAACTC106950.06.41421270
ACGTCTG147450.06.385215370
AGTCACA51700.06.295938570
AACTCCA92600.06.27429870
GAACTCC93350.06.26138270
CGTCTGA136500.06.17948770
CACGTCT164550.06.104527570
Produced by FastQC (version 0.11.5)