FastQCFastQC Report
Fri 19 Oct 2018
000000000-BNG4D_l01_n01_abdna_ablib_dapv2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNG4D_l01_n01_abdna_ablib_dapv2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4680342
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT5257.2759576E-1111.99999951
TATGCCG6452.5975169E-99.76744248
TGCCGTC7001.01270416E-78.550
ATGCCGT8201.803528E-88.10975649
CCAGTCA55150.07.552130770
GTCGGCA6108.863947E-57.45901645
GTATGCC9051.0302938E-77.348066347
TCCAGTC59850.07.01754469
AACTCCA94850.06.826568670
GAACTCC94500.06.81481569
CGTATGC10106.9691305E-76.584158446
CTCCAGT68300.06.35431970
ACGTCTG147850.06.320595770
CAGTCAC47750.06.30366569
TTACTCG27350.06.270566569
TGAACTC108300.06.204986670
CGTCTGA136650.06.198317570
ACTCCAG72850.06.05353569
CACGTCT164350.06.02677270
CTAAAGG14609.906216E-95.99315071
Produced by FastQC (version 0.11.5)