FastQCFastQC Report
Thu 17 May 2018
000000000-BNDCH_l01n01.331000000dca3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BNDCH_l01n01.331000000dca3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17519424
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC2838881.6204185708388588RNA PCR Primer, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC332300.041.7296145
CGTATGC341950.040.5585644
TCGTATG348050.039.82186543
CTCGTAT344950.039.76880642
TCTCGTA356200.038.27905741
TATCTCG368700.037.1338539
ATCTCGT383100.035.6675840
ATATCTC397350.034.45073738
CAATATC432500.031.70809436
AATATCT437750.031.33295237
CCAATAT495450.027.89736635
GCCAATA509050.027.31558834
CGCCAAT527350.026.47435233
ACGCCAA563550.024.80170232
CACGCCA603650.023.18023731
TCACGCC620350.022.51994730
GTCACGC659300.021.2270629
AGTCACG708800.019.77638228
CAGTCAC782700.017.91203527
ACACCTG52250.016.622016
Produced by FastQC (version 0.11.2)