FastQCFastQC Report
Thu 21 Jun 2018
000000000-BMMKK_l01n03.333000000ea1e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMMKK_l01n03.333000000ea1e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16247902
Sequences flagged as poor quality0
Sequence length251
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG13903668.557203262304265No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG11799437.262125288544946No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA10200686.278152096190634No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG5616393.4566862847892605No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG3968882.4427030640632865No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC3363432.0700703389274504No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA2755071.6956466133289085No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2628671.6178519540553604No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA2328461.4330834836399184No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG2148591.3223799601942454No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1930551.188184172947375No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1906051.1731053030723597No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1805921.1114788850892872No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1785571.0989541911318765No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1782561.0971016442615176No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA1630841.0037234345701986No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1449770.892281354232688No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA1423770.8762792882428758No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC1049110.6456895173296836No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG1011040.6222587999361395No Hit
CCTGTTCGCTACCCACGCTTTCGTTCCTCAGCGTCAGTTACTGCCCAGAG960640.5912394104789652No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG902300.555333236254133No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGACCAGTA872460.5369677882104409No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG844220.5195870826891988No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC840360.5172113913537884No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGAA820120.5047543984447961No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT747560.46009632505168974No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA747130.45983167549878134No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA703420.4329297407136011No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA695550.4280860384313002No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG603530.371451034108896No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA587140.3613635778945491No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA578620.35611982396250297No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG553650.34075168597151806No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTTCTGTCCAGTG523870.32242316577241786No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG521990.32126609330853917No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAA513570.31608388578414615No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAG491230.30233441831443836No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATTAGTCCAGGT486930.29968792278535406No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA474170.29183460116881554No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA459570.28284882565145947No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT448210.27585715374206465No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA446690.2749216483457372No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG432560.26622514094435085No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA408120.2511831989139275No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA404810.24914601282060908No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGGCCCAGGG387880.2387262060049353No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA386520.23788917486085281No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG358250.22049000541731478No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATGAGCCAGTG347190.21368297273087933No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG325280.20019815481408No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATTCGTCCAGTA323420.19905339163173189No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA320590.1973116282951485No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA307690.18937214170789557No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG276530.1701942810831823No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA276470.17015735323859044No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA262070.16129467053654065No Hit
ACTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG244420.15043172958576437No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA232730.14323695453111424No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA232420.14304616066738954No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGACGGAGCCTGGTA217560.1339003644901354No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA216350.13315565295753262No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT205400.1264163213195156No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA198690.122286557365991No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTTGCGATACGGTA184040.1132700086448084No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATGAACGTCAGTGTTATCCCAGGA180590.11114665758077567No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC173940.10705382147184295No Hit
ACTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG170650.10502894466005519No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGATGGTCCAGTT166280.10233936664561369No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTATGATGCCAGGG164280.10110843849255122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC8741950.0244.845873
CTGTTCG2305500.0244.697142
TTTGCTC6978400.0244.650685
TGTTCGA628650.0244.181583
CCTGTTC2264000.0244.139711
TTGCTCC7052200.0244.101946
TGTTTGA1304000.0243.994843
GTTTGCT8875550.0243.491464
CTGTTTG10053300.0243.442762
CCGTTCG1388800.0243.191792
TTGATAC958750.0242.16356
TTTGATC365900.0241.585145
CTCCCCA7938800.0241.449439
GCTCCCC9879150.0241.205668
CCTGTTT9984350.0240.937651
TGTTCGC1716150.0240.602953
CGTTCGC1403200.0240.329423
TGCTCCC7167100.0239.21617
TTTGCTA1985600.0238.337025
TGCTACC1998100.0237.814687
Produced by FastQC (version 0.11.2)