FastQCFastQC Report
Thu 21 Jun 2018
000000000-BMMKK_l01n02.332000000ea1e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMMKK_l01n02.332000000ea1e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16247902
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGACAAGGGA3094891.9047936158157526No Hit
TAGCTCGTAACT1724041.0610846865029098No Hit
CGGAGCTATGGT1556910.9582221753922445No Hit
AAGGAGCGCCTT1453910.8948293755095273No Hit
ACGTGTACCCAA1448220.8913273849140646No Hit
TACACGATCTAC1369920.8431365477216689No Hit
TAAAGTCACCCT1348110.8297132762125227No Hit
GAAGTTGGAAGT1340520.8250419038716506No Hit
CAACGGGTAGTC1336800.8227523775069544No Hit
TCCTCTGTCGAC1334100.82109062450032No Hit
TACAGATGGCTC1320390.8126526120110769No Hit
GACTTTCCCTCG1315170.8094398895315837No Hit
GTTGGTCAATCT1270240.7817870885730355No Hit
ATCCCGAATTTG1260000.7754847364293556No Hit
ACGCGCAGATAC1225550.7542819989928545No Hit
GAGGCCATCAGT1207700.743295965226772No Hit
TTCCTAGGTGAG1180580.726604579471245No Hit
TGAGTCACTGGT1169730.719926794240881No Hit
GAGTGGTAGAGA1142120.7029338310878537No Hit
CCTCGTTCGACT1138100.7004596655001981No Hit
CCTAATGGAACC1134920.6985024897368287No Hit
CACGGTTGTGAG1096220.67468402997507No Hit
CACGCCATAATG1073540.6607253047193417No Hit
GAGGCTCATCAT1064870.6553892311758158No Hit
ACCCGTATGATG1057030.6505639928158109No Hit
CCAGTGTATGCA1055910.6498746730500959No Hit
GACTTGGTATTC1052830.6479790436943798No Hit
TGACCGGTCAAT1048000.6450063522047339No Hit
ACGGGACATGCT1038160.6389501856916665No Hit
AATCAGTCTCGT1034740.6368452985499297No Hit
GGCCACGTAGTA1018530.6268686258693584No Hit
CAGCGGTGACAT1017370.6261546875405821No Hit
CACATGCCTAAG1012850.623372789914661No Hit
CCTGAACTAGTT1011590.6225973051782316No Hit
CTTCGGCAGAAT999650.6152486641044487No Hit
TGCGGCATCGAA996170.61310684911812No Hit
AATCCGTACAGC985410.6064844556546439No Hit
TGTTTGAGCTGT977260.6014684234309143No Hit
TGAGCCGGAATC975780.6005575365976481No Hit
GTAAGCACCTAC968360.5959907931497863No Hit
TCCCAGAACAAC957780.5894791832200859No Hit
CAGGCGTATTGG957560.589343781123249No Hit
CTGGAAATCTGC948550.5837984497937025No Hit
TAGGAACTGGCC948040.5834845631146717No Hit
CTCCTGAAAGTT945090.5816689440889046No Hit
GACTGATCATCT938250.5774591698054309No Hit
TACCTCTCAGAA938170.5774099326793083No Hit
ATTCCTGTGAGT933860.5747572825094588No Hit
GTCGTCCTAAAT933070.5742710658889991No Hit
ACACGTAAGCCT929740.5722215705141501No Hit
CGTTTAGAGTCG923470.5683626107542993No Hit
GGTAACAGCTCG922750.5679194766191967No Hit
CTGTAGGAGACC916660.5641713003931215No Hit
TACGTCCCGTTC915070.5631927125114369No Hit
TACGATGACCAC905740.5574504326774005No Hit
CTAGCGAACATC896160.5515542868242312No Hit
CCTCTACCTACG895970.5514373486496903No Hit
GCACTACCGAAT887360.5461382029507564No Hit
CCTTGGCTATCC884570.5444210581772342No Hit
CAAGCATGCCTA880600.5419776657934052No Hit
TCCAAAGTGTTC860300.5294837450398211No Hit
TTACTGTGCGAT857890.5280004766153809No Hit
ACACGAGCCACA851640.5241538261370606No Hit
AGAACACGTCTC849600.5228982794209369No Hit
GACGGAACCCAT848830.5224243720820079No Hit
GTCAATTGACCG847140.5213842377926701No Hit
ATGTGCACGACT844640.5198455776013421No Hit
TTCGCCCTTCAG839030.5163928241320017No Hit
ACACAAAGGGAG837180.5152542155904191No Hit
TTCTGGGAACAC835830.5144233390871018No Hit
TAACTCTGATGC835010.5139186585443463No Hit
CTTGCTGAAGAC831440.5117214517911297No Hit
GCACACACGTTA829360.5104412865119448No Hit
CTGCTAACGCAA827490.5092903686888313No Hit
GCGATATATCGC825030.5077763270605644No Hit
ACACCTGGTGAT822700.5063422957622468No Hit
GATAGAAAGCCA822460.5061945843838792No Hit
TACCCAGAGATC821090.5053513985990314No Hit
GAGGAATAGCAG816530.502544882410049No Hit
TAGGATTGCTCG812820.5002615106861181No Hit
CGAGATACCCAG810760.4989936546884638No Hit
TCGAGGACTGCA808720.4977381079723401No Hit
GTGCCGGTGATA807030.4966979736830023No Hit
AAGCCTACACGT805820.4959532621503995No Hit
AGGCATCTTACG804130.49491312786106173No Hit
TCTAGCGTAGTG802250.493756055397183No Hit
ATGTACGGCGAC798120.49121418876110895No Hit
CAGCATGTGTTG796560.4902540648017203No Hit
ATGGATACGCTC793960.488653858202739No Hit
TGATTTGGACCT793560.4884076725721265No Hit
TGGAGCACGTTG790610.48659205354635937No Hit
AAGGTGTAAGGT786780.48423482613324476No Hit
CGATTTCTCAAG784250.48267770201962074No Hit
GTACATACCGGT783470.4821976400399264No Hit
AAGTGGACTCTC783300.48209301114691605No Hit
GCTAATTACGCT778880.4793726599286481No Hit
TAGAACTCACCT771120.47459665869476564No Hit
TGCTATATCTGG759190.46725417226174804No Hit
TGAGTTCGCTAT755550.46501388302317437No Hit
CTAACCTCCGCT752260.46298900621138656No Hit
ATCTCTGGCATA751100.4622750678826103No Hit
AGGCTACACGAC749470.4612718614378644No Hit
TGGACACCGAAC736740.4534370037436218No Hit
GAACTAGTCACC729490.4489748891887703No Hit
CGCAAATTCGAC722500.44467279529381704No Hit
TCGCATGAAGTC718390.4421432379392736No Hit
AGATAGGACAGG715580.4404137838842209No Hit
CGCACATGTTAT705540.43423452455584727No Hit
ATTCTGTGAGCG701450.4317172764828345No Hit
GCGTGTTAAACC698760.4300616781169655No Hit
GATAGCTGTCTT692200.4260242337749206No Hit
TCCAGGCTTAAT689420.4243132436421638No Hit
TATCGTTGACCA686620.4225899442278763No Hit
CATCCAAATGCG685130.4216729027538448No Hit
CCATAATCCGTA682000.41974650019430204No Hit
AGCTGGAAGTCC678490.41758622128567735No Hit
GGTAGGAACAAT661290.40700023916934014No Hit
TAAGGCCTATCG660140.40629245548132925No Hit
CCATTCGCCCAT658860.4055046614633693No Hit
GACAGGAGATAG657340.4045691560670418No Hit
ATTCATGGACGC639790.39376776152391857No Hit
TCCGAATTCACA626450.38555747074299196No Hit
AATGCCTCAACT623630.38382186204717383No Hit
ATTATACCTCGG622560.3831633154852854No Hit
AGTTTGCAACGC613680.377697994485688No Hit
GAATCTTCGAGC591630.36412701159817434No Hit
GTCATATCGTAC589860.36303764018271406No Hit
TTATCACGTGCA585120.360120340459956No Hit
CATTTGGACGAC568930.35015597706091534No Hit
CAGCTAGAACGC562580.34624778017494195No Hit
GCGACTTGTGTA562510.3462046976895848No Hit
ACTGACAGCCAT552270.3399023455459049No Hit
ACGTGAGAGAAT545810.33592644761151313No Hit
TGTGAGCACGGT542400.33382771511054166No Hit
GTTACGCATTAC534600.32902709531359803No Hit
TTCCGTAGGGAT530840.3267129503858406No Hit
TGAGGATGATAG513400.3159792568911358No Hit
CGTAACCAACCA503760.3100461831933748No Hit
AGCTGTTGTTTG501770.30882140968107763No Hit
TTGCAGACAGGC480800.2959151279962176No Hit
CACGAGGTCATT467030.2874401876623825No Hit
ACCGGCTAGAGT433610.2668713782247087No Hit
CTGGCCTAGGAA420400.25874109777373105No Hit
ATGAGACTCCAC405910.24982302330479345No Hit
ACTTATGCGGTT392750.2417235160576424No Hit
CAAGTTTACGGC385830.23746450464804625No Hit
AAGAGATGTCGA369640.22750014124900556No Hit
TTACGAGACGGC355030.2185082110908842No Hit
GTCATTCACGAG312160.1921232661299902No Hit
GCCAATATCTCG204110.1256223726607903Illumina PCR Primer Index 6 (100% over 12bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT953.4298973E-66.02
TGCCCGG751.7513822E-46.01
ATAGGCC704.69743E-46.03
GGTCGAG650.00126198066.05
ACGTTGG1451.9645086E-106.02
CCGATGG650.00126198066.03
GCGCGCC1259.677024E-96.04
CGTTGGA1451.9645086E-106.03
GGTCAGG550.0091607616.06
CACTGGG550.0091607616.06
ATGTCGC600.00339658956.06
GTCAGGT600.00339658956.06
GGTGCGC806.539458E-56.03
CGTCGTG704.69743E-46.06
CCGGTGC650.00126198066.04
GGCGCGC1351.375156E-96.03
CGCCGGA1303.6470738E-96.03
TCAAGGG1101.8153878E-76.05
TCTGGGT600.00339658956.02
GCCGTAA704.69743E-46.04
Produced by FastQC (version 0.11.2)