FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818183
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG215550.0144.831833
ACACGAT157550.0144.539833
CGCGCGG215850.0144.496194
ATCGCGC216450.0144.430592
TACACGA157900.0144.311282
ATCGTCT221650.0144.28049
CACGATC157750.0144.21874
ATACACG157300.0144.170381
TCGTTAC154800.0144.110145
ACGATCT157750.0144.080835
AAGTAGA199300.0143.945072
TAAGTAG199200.0143.944531
CATGATC222550.0143.89245
AGAGTCT198850.0143.723926
CGGTTCT215300.0143.31638
TGATCGT223250.0143.311317
AGTCTTG199950.0142.96958
CGCGGTT216350.0142.922356
GATCATG224650.0142.740942
GATCGCG218400.0142.377521