FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_PDL1.H.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_PDL1.H.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429626
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC21320.14912991229874106No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT19420.13583972311639547No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG18230.1275158677864001No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT16320.11415573023993689No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT14910.10429301089935411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC202.1309243E-6145.02
ACGTTAC151.2301152E-4145.04
GCGCGAT700.0145.05
GGTTTTG100.0070911786145.09
ATCGCGG151.2301152E-4145.02
TCGCGTG306.511982E-10145.03
GATCGCG309850.0144.976611
TACACGA281750.0144.92282
ATCGCGC310000.0144.906452
TCGCGCG309400.0144.85943
ACACGAT282150.0144.820133
ATACACG282350.0144.76891
CACGATC281900.0144.768544
ACGATCT281700.0144.742655
GCGCGGT307700.0144.599445
ATGATCG321300.0144.571276
GCGGTTC307300.0144.551767
ATCGTTA256700.0144.519874
TCGTTAC256450.0144.491135
ATCGTCT322150.0144.324849
Produced by FastQC (version 0.11.5)