FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_PDL1.G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_PDL1.G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441914
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT80490.5582163707405574No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG69310.4806805398935026No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC66320.4599442130390578No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT64610.44808497594169977No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT58850.4081380720348093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC550.0145.000022
TCTCGCG550.0145.000023
TCCGCGG100.007091184145.03
GCGGTCC253.7123755E-8145.07
GCGCGAT600.0145.05
GATCCGC100.007091184145.01
TTCACGA306.511982E-10145.02
ATAGCGG100.007091184145.0145
GGTTTTG100.007091184145.09
GCGCCGT100.007091184145.05
CACGATT151.230117E-4145.04
ATCCGCG100.007091184145.02
ACGATCC202.1309297E-6145.05
ATTCACG306.511982E-10145.01
AACGCGC151.230117E-4145.02
TACCTCT151.230117E-4145.09
TTGCGCG351.0913936E-11145.03
ATTGCGC351.0913936E-11145.02
TATACGA151.230117E-4145.02
TTCGCGC100.007091184145.02
Produced by FastQC (version 0.11.5)