FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_PDL1.F.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_PDL1.F.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129255
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC18680.16541879380653615No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT17930.15877724694599538No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG16490.14602547697375703No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT16370.1449628294760705No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT13560.12007916723857764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG151.2300472E-4145.03
TCGTTCG306.511982E-10145.01
CACGGTC100.007090983145.04
TACGCGA100.007090983145.02
ATTCACG151.2300472E-4145.01
TCGCTCG306.511982E-10145.03
TCGTCAC100.007090983145.05
CTCGCGG151.2300472E-4145.04
ATTATCG100.007090983145.06
TTCGCGC202.1307278E-6145.02
ATCGCGG100.007090983145.02
TCGCGTG306.511982E-10145.03
GATCGCG243150.0144.940371
ATACACG225400.0144.871341
ATCGCGC243350.0144.851042
TCGCGCG242950.0144.820953
TACACGA225650.0144.775092
ACGATCT225600.0144.646485
GCGCGGT241450.0144.639685
ATGATCG242750.0144.611746
Produced by FastQC (version 0.11.5)