FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_PDL1.E.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_PDL1.E.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319655
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT31550.23907763771591817No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT30190.2287719138714285No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC28340.21475309834767423No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG26040.19732430066949314No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT25340.19201988398482936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCC351.0913936E-11145.07
GCGCGAT351.0913936E-11145.05
CGCGATT351.0913936E-11145.06
GCGTTCT151.2300936E-4145.07
TACGCGA151.2300936E-4145.02
TCTCGCG306.511982E-10145.03
TTGCGCG351.0913936E-11145.03
CGCGTTC151.2300936E-4145.06
ATCGGCG151.2300936E-4145.02
AACGCGC100.0070911185144.999982
CTCGATC202.1308624E-6144.999984
ATCGCGG202.1308624E-6144.999982
ATCGCGC306850.0144.976362
GATCGCG306450.0144.952681
TCGCGCG306600.0144.905433
TACACGA231400.0144.874682
ATACACG231750.0144.843571
ACACGAT231450.0144.843383
CACGATC231800.0144.687234
ATGATCG249100.0144.592546
Produced by FastQC (version 0.11.5)