FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_PDL1.D.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_PDL1.D.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468836
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC28550.19437159764602718No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT27840.18953783812488256No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT26360.17946183236249655No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG23010.1566546571570958No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT21640.14732754371488715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT306.511982E-10145.05
TTCACGA151.2301217E-4145.02
ACGATCC351.0913936E-11145.05
ATCGCGG253.7123755E-8145.02
TCGCGGG202.1309443E-6145.03
GATCGCG309850.0144.97661
TACACGA257000.0144.887162
TCGCGCG310150.0144.883133
ACACGAT257250.0144.83093
ATCGCGC310800.0144.790052
ATACACG257300.0144.746411
CACGATC257300.0144.690054
GCGCGGT308800.0144.671315
GCGGTTC309200.0144.577967
ATGATCG313200.0144.467596
ATCGTCT313450.0144.444899
ACGATCT257600.0144.408975
TGATCGT313500.0144.352487
CGATCAT314150.0144.330731
TCGTTAC252500.0144.253465
Produced by FastQC (version 0.11.5)