FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_PDL1.C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_PDL1.C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956094
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC49600.2535665463929648No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT44840.22923233750525282No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT44100.22544928822439003No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT41910.21425350724453934No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG41470.21200412659105342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC306.511982E-10145.02
GCGCGAT351.0913936E-11145.05
GACCGTC100.0070913765145.08
ATCGTAC151.2301838E-4145.04
ACGATCC500.0145.05
TACACGA352750.0144.91782
ATACACG352650.0144.89721
ATCGCGC409150.0144.840532
GATCGCG408900.0144.82271
TCGCGCG408350.0144.822453
CACGATC352650.0144.75334
ACACGAT353200.0144.69213
GCGCGGT407600.0144.484185
ACGATCT353300.0144.466465
TAAGTAG382200.0144.449891
GCGGTTC408150.0144.37837
TGATCGT428350.0144.373767
TCGTTAC359650.0144.354925
CATGATC428900.0144.306955
CGATCGT359750.0144.294652
Produced by FastQC (version 0.11.5)