FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_PDL1.B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_PDL1.B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761158
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT40250.22854281103682916No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT34390.19526924898277156No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC32830.18641144065438764No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT26210.14882253608137372No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG25250.14337157711006054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC306.511982E-10145.02
ACGATAT151.2301632E-4145.05
TACGCGA151.2301632E-4145.02
AACGCGC202.1310625E-6145.02
TCGTCAC151.2301632E-4145.05
CTCGCGG306.511982E-10145.04
ATGCGCG151.2301632E-4145.02
ATCGCGG100.0070913173145.02
TCGCGTG253.7127393E-8145.03
GATCGCG385600.0144.887191
ATCGCGC386000.0144.849752
TACACGA271950.0144.840042
TCGCGCG385500.0144.811943
ATACACG272050.0144.760151
GCGCGGT383950.0144.565695
ACACGAT272450.0144.547623
TCGTTAC282100.0144.486015
TAAGTAG450700.0144.453081
GCGGTTC383500.0144.451757
ATCGTTA282750.0144.384634
Produced by FastQC (version 0.11.5)