FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_PDL1.A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_PDL1.A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695571
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC50030.29506284313661885No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT45490.2682872023642773No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT40600.23944736021080804No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT36750.21674114501840383No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG32650.1925605002680513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT351.0913936E-11145.05
GACCGTC253.7125574E-8145.08
TCGATCC100.0070912936145.01
TCGTGCG400.0145.03
TCGCTCG750.0145.03
ATCGGCG100.0070912936145.02
ATCGCGC323050.0144.910232
TCGCGCG322100.0144.909973
GATCGCG322700.0144.887661
TACACGA259400.0144.832292
ATACACG259650.0144.692861
GCGGTTC320300.0144.660487
GCGCGGT320800.0144.57065
TCGATCG366850.0144.52571
TCGTTAC367100.0144.466775
ACGATCT260000.0144.44235
ACACGAT260000.0144.414433
ATCGTTA367350.0144.388184
CGATCAT409300.0144.362341
CACGATC260100.0144.331024
Produced by FastQC (version 0.11.5)