FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_PDL1.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_PDL1.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196472
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT31370.2621874979105236No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC28370.2371137811833457No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT26830.22424260659672773No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG24420.20410005415922813No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT23190.1938198303010852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC151.2300654E-4145.02
CGCGGTC306.511982E-10145.06
GCGGTCT253.7118298E-8145.07
GCGCGAT306.511982E-10145.05
TCGCTCG253.7118298E-8145.03
ATCGCTC253.7118298E-8145.02
ATCGCAC306.511982E-10145.02
TCGTTCG100.007091036144.999981
TCGATCT202.1307806E-6144.999981
CCCGCTC100.007091036144.999984
GATCGCG237600.0144.938961
TACACGA212900.0144.93192
ATACACG212900.0144.897841
ACACGAT212950.0144.795733
ATCGCGC238300.0144.75662
TCGCGCG238100.0144.634613
ATGATCG244200.0144.614046
TCGTTAC210000.0144.585715
GCGCGGT236700.0144.571185
ACGATCT213600.0144.490885
Produced by FastQC (version 0.11.5)