FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_PDL1.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_PDL1.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413681
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT37210.2632135538356956No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT32420.22933037934300596No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC29180.20641148887195912No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG27820.19679121385942092No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT26060.18434144619613618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCTC550.0145.000022
ATCTCGC253.7121936E-8145.02
GCGCGAT151.2301121E-4145.05
TCGTCAC151.2301121E-4145.05
CTCGCGG253.7121936E-8145.04
CTCGATC253.7121936E-8145.04
TACACGG306.511982E-10145.02
TCTATCG306.511982E-10145.01
GATCGCG290000.0144.9751
TACACGA253100.0144.971362
ATCGCGC290050.0144.900012
ACACGAT253300.0144.856893
TCGCGCG289800.0144.849883
ATACACG253700.0144.714231
CACGATC253850.0144.685844
ACGATCT253200.0144.65645
GCGCGGT289100.0144.648915
TCGATCG242650.0144.551821
ATCGTCT270400.0144.490579
TCGTTAC242700.0144.432435
Produced by FastQC (version 0.11.5)