FastQCFastQC Report
Tue 5 Feb 2019
000000000-BMH7K_l01_n01_PDL1.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH7K_l01_n01_PDL1.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407267
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC32620.2317968089921813No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT29020.20621530953259049No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG26640.1893030960009721No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT26450.1879529613072715No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT21440.15235204122600757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC151.230111E-4145.02
ACGATCC253.7121936E-8145.05
TCGCTCG351.0913936E-11145.03
CTCGCGG151.230111E-4145.04
GATCCGC100.0070911674144.999981
TCGGCGG100.0070911674144.999983
ATCCGCG100.0070911674144.999982
TTCGCGC100.0070911674144.999982
ATACCGA100.0070911674144.999981
TCGCGGG202.1309133E-6144.999983
TACACGA260050.0144.916372
ATACACG260000.0144.916351
ATCGCGC312450.0144.883992
TCGCGCG312250.0144.837463
TCGTTAC210000.0144.689295
ATGATCG327350.0144.534916
GCGCGGT310800.0144.533465
ACACGAT260750.0144.527313
ATCGTTA210600.0144.518044
CACGATC260800.0144.49964
Produced by FastQC (version 0.11.5)