FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11636895
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC792600.6811095227721828No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT569400.4893057813102206No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT537820.4621679580334789No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC523760.45008569725859No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC475870.4089321077486735No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC450680.3872854399734637No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC432530.3716884959432907No Hit
AGGAATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT421900.36255375682258884No Hit
CTAGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT384460.3303802259967113No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT383030.3291513758610007No Hit
ATCTTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT382380.32859280761749593No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT376120.32321336576466486No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC364830.3135114650428658No Hit
AGGAAGGACTACGCGGCTGCTGACACTTGACCGACTGTCTCTTATACACA299620.257474180182944No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG145090.12468102530786777No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT134500.11558065961753544No Hit
CGATTCCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC129580.11135272768208358No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG126600.1087919071195538No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC116920.1004735369701282No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT116480.1000954292360634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCC276950.0274.38621
GATTCCT263800.0267.498632
CCACGTC253150.0261.75041
ATCCTAT231250.0261.330752
TCCTATT199850.0260.730963
CTCAGCT208750.0257.08913
ATTCCTA279300.0255.391243
CCTAGAA303150.0253.323496
GCGAAGT181100.0252.864671
TCTCAGC271250.0252.181182
CGTCTAG221250.0251.953574
GAGACTT151000.0244.445791
CTAGGTA185800.0243.75011
TCTGTAG241700.0242.243212
AATCCTA247850.0241.098331
TCAGCTA229850.0240.989534
AGGAAGG313450.0239.212661
CACGTCT229550.0238.85522
TAGGTAG204050.0238.28812
CTTATAG204400.0235.987533
Produced by FastQC (version 0.11.5)