FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-99-4-7-5-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-99-4-7-5-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22220
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC450820.288028802880287No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT256111.525652565256525No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCGCTTCTG16077.232223222322231No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC13436.044104410441044No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCGCTT10104.545454545454546No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCGCT6552.947794779477948No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT6492.920792079207921No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC4321.9441944194419443No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCGCTTCT4201.8901890189018902No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC4031.8136813681368138No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCGCT3551.5976597659765976No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT3251.4626462646264626No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCGCTTC2641.188118811881188No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCGCTT2561.1521152115211521No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCG2291.0306030603060305No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCGCT1940.8730873087308731No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA1850.8325832583258326No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCGCTT1790.8055805580558055No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1750.7875787578757876No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1650.7425742574257426No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCG1600.7200720072007201No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTC1550.6975697569756976No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCGC1550.6975697569756976No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1320.594059405940594No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCGCT1300.585058505850585No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTC1290.5805580558055806No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCGC1290.5805580558055806No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG1240.5580558055805581No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTC980.44104410441044106No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG980.44104410441044106No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT970.4365436543654366No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG950.42754275427542754No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTCGCT860.387038703870387No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGT850.38253825382538253No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCGCTT840.37803780378037805No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG760.342034203420342No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCGC750.33753375337533753No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTCG690.31053105310531054No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT670.30153015301530156No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTC650.2925292529252925No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTCG610.2745274527452745No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTC610.2745274527452745No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC610.2745274527452745No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA590.26552655265526554No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTCGCTT550.24752475247524752No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCGCTT540.24302430243024303No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG540.24302430243024303No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC520.234023402340234No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC510.22952295229522954No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTC510.22952295229522954No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCGCTT470.2115211521152115No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGT440.19801980198019803No Hit
GCGAAGTTAGAACAGGCTCCTCTATGTCTCGGGGGGTATCTAATCCCAGT440.19801980198019803No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA430.1935193519351935No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGCTGGGGTATCTAATCCCAGT430.1935193519351935No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGTCAGGGGTATCTAATCCCAGT410.1845184518451845No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTC400.18001800180018002No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTCGCT370.1665166516651665No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCAG370.1665166516651665No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCACGTGGGCTCGGAGGGGTATCT350.15751575157515751No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC350.15751575157515751No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCGC350.15751575157515751No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTCG350.15751575157515751No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGTCGGCATCAGGGGTATCTAATC330.1485148514851485No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC330.1485148514851485No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGGTATCTAATCCCAGTCG330.1485148514851485No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCATGGGGTATCTAATCCCAGT320.14401440144014402No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG310.13951395139513953No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGTGGGGTATCTAATCC310.13951395139513953No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCGC310.13951395139513953No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGAAGCGTCAGGGGTATCTAATCCCA300.135013501350135No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC280.126012601260126No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT270.12151215121512152No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA270.12151215121512152No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGCTCGGGGTATCTAATCCCAG270.12151215121512152No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCGGGGTATCTAATCCCAGT250.11251125112511251No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA250.11251125112511251No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTC240.10801080108010801No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT240.10801080108010801No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGT230.10351035103510352No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTAAGCTTGGAACAGGGCGGGGGGT230.10351035103510352No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC230.10351035103510352No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC230.10351035103510352No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTTGA108.409296E-4295.04
CGAAGTT22000.0294.329562
GCGAAGT22100.0293.665161
GAAGTTA22000.0292.988653
AAGTTAG22050.0292.324254
AGTTAGA22050.0292.324255
GTTAGAA21950.0292.312076
TTAGAAC22000.0291.647747
AGAACAG23000.0276.40229
TAGAACA23150.0275.248388
GTTGAAC150.0028309827196.666676
GTTAGAC150.0028309827196.666676
AGTTGAA150.0028309827196.666675
TTGAACA150.0028309827196.666677
TAGAACG255.483807E-5177.08
AGAACGG352.0953738E-4126.4285669
CGTCAGG150.00437010359.00000425-29
TGTTGAA303.3149445E-759.000004105-109
CCGTGTC150.00437010359.000004215-219
GTTTGGG600.059.00000430-34
Produced by FastQC (version 0.11.5)