FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-98-4-1-3-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-98-4-1-3-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22138
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT355916.0764296684434No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTA248811.238594272291987No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATCT19918.993585689764206No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT11735.29858162435631No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTA8763.9569970187008767No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCGA8343.7672779835576833No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGATC5892.66058361188906No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACCG5762.6018610533923567No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTA4011.811365073629054No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCGA3471.5674405998735208No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAAC3441.5538892402204356No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT3441.5538892402204356No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT3441.5538892402204356No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGAT3401.5358207606829886No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACCG3141.4183756436895836No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTA2961.3370674857710725No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT2611.1789682898184117No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2601.17445116993405No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAACC1960.8853554973348993No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1830.8266329388381968No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTAA1810.8175986990694732No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT1470.6640166230011745No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA1400.6323967838106423No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT1280.5781913451983016No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACCG1190.537537266239046No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG1160.5239859065859608No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1150.5194687867015991No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCGA1030.46526334808925823No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTAA1030.46526334808925823No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTAAC1030.46526334808925823No Hit
TGCTTATAGAACAGGCTCCTCTAGTGTTAGGGGGGTATCTAATCCCAGTA990.44719486855181134No Hit
TGCTTATAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGTAA900.4065407895925558No Hit
TGCTTATAGAACAGGCTCCTCTAGCTTGGGGTATCTAATCCCAGTAACCG880.39750654982383227No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT790.3568524708645768No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCGAT780.352335350980215No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT770.3478182310958533No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTA720.3252326316740446No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCGA690.3116812720209594No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC680.3071641521365977No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACCGA640.2890956725991508No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTAACC620.28006143283042734No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT610.2755443129460656No Hit
TGCTTATAGAACAGGCTCCTCTAGTGTCAGTGGGGTATCTAATCCCAGTA610.2755443129460656No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACCGA590.26651007317734215No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTAA590.26651007317734215No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCGA580.2619929532929804No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAACC560.2529587135242569No Hit
TGCTTATAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACCG560.2529587135242569No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGCGGGGTATCTAATCCCAGTA530.2394073538711717No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA530.2394073538711717No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTAA530.2394073538711717No Hit
TGCTTATAGAACAGGCTCCTCTAGGTATGCAGCGTGTGGAGCAACGGTTG520.23489023398681003No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCT500.22585599421808653No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGTA490.22133887433372482No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT480.21682175444936308No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTAA480.21682175444936308No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT460.20778751468063963No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTAAC430.19423615502755445No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAACC420.1897190351431927No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTAA400.18068479537446924No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTAA400.18068479537446924No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAG380.17165055560574577No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA370.16713343572138406No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTTCGTGGGGTATCTAATCCCAGTA360.1626163158370223No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTAACCG360.1626163158370223No Hit
TGCTTATAGAACAGGCTCCTCTAGCTCTCTGAGGTTCCTGGGAGGTCGTC360.1626163158370223No Hit
TGCTTATAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTAACCG360.1626163158370223No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGCTTGGGGTATCTAATCCCAGTA350.1580991959526606No Hit
TGCTTATAGAACAGGCTCCTCTAGTGCTGTTATAAAATCACCATCATGTG340.15358207606829885No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTAACCGAT330.14906495618393711No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCAGT300.13551359653085193No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCCTGGGGTATCTAATC280.12647935676212846No Hit
TGCTTATAGAACAGGCTCCTCTAGACGTGGGGTATCTAATCCCAGTAACC280.12647935676212846No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA270.12196223687776674No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTA260.11744511699340501No Hit
TGCTTATAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTAACCG260.11744511699340501No Hit
TGCTTATAGAACAGGCTCCTCTAGATCTCGTGGGCTCGGGGTATCTAATC240.10841087722468154No Hit
TGCTTATAGAACAGGCTCCTCTAGCCATAAAATCCGTGGTTTTGCTATCA230.10389375734031982No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT230.10389375734031982No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGTGGGGTATCTAATCCCAGTA230.10389375734031982No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT230.10389375734031982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA21900.0294.326482
CTTATAG21850.0293.64993
TGCTTAT22200.0293.006741
TTATAGA22100.0290.328064
GAACAGG21900.0288.264839
ATAGAAC22050.0286.972786
TATAGAA22300.0286.401345
TAGAACA22600.0279.988927
AGAACAG22850.0276.92568
CTTATAA150.0028308963196.666673
GCTTATG304.5682464E-7196.666672
CTTATGA359.846262E-7168.571433
ACAGCCG200.0066933595147.5295
TTATGAA453.4400855E-6131.111114
GCAAGAG455.6948315E-498.33333295
TGAACAG652.1390722E-590.769237
TATGAAC703.089849E-584.285715
TCCTTTG6650.059.00000445-49
GAGCGGT501.8189894E-1259.00000485-89
GGTTGGG257.787667E-659.00000420-24
Produced by FastQC (version 0.11.5)