FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-97-4-1-3-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-97-4-1-3-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22039
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC355316.121421117110575No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG295813.421661599891102No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGAT19959.052134851853532No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACC10054.560098008076592No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC9934.505649076636871No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG5632.554562366713553No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG5322.413902627160942No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGA4932.23694359998185No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACC3971.8013521484640864No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAAC3921.7786650936975363No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAAC3911.774127682744226No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA3621.6425427650982352No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC3331.5109578474522438No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC3301.4973456145923136No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT3031.3748355188529424No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT2491.1298153273742002No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCG2130.9664685330550389No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT2050.9301692454285585No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA1890.8575706701755978No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACC1840.8348836154090477No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1750.7940469168292573No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT1620.7350605744362266No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA1580.7169109306229865No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA1370.6216253006034757No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT1330.6034756567902355No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTA1240.5626389582104452No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAAC1230.5581015472571351No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC1180.5354144924905849No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGC1150.5218022596306547No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTA1090.49457779391079454No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1020.4628159172376242No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTA1010.4582785062843142No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCAACTAGGGGTATCTAATCCCAG980.4446662734243841No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA970.440128862471074No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTAACC930.4219792186578339No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC920.4174418077045238No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT870.39475475293797363No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAA860.39021734198466357No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCG860.39021734198466357No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTTGGGGTATCTAATCCCA830.37660510912473344No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACC790.35845546531149325No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG780.35391805435818324No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAG750.3403058214982531No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC740.33576841054494305No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCCTGGGGTATCT700.3176187667317029No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA670.3040065338717728No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACC570.25863242433867234No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACC550.24955760243205227No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT520.23594536957212217No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAAC510.2314079586188121No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG470.21325831480557195No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC450.20418349289895188No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTAAC440.19964608194564185No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCCGCACGTCAGGGGTATCTAATC440.19964608194564185No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCA420.19057126003902172No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC400.18149643813240166No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGCGGCAGGTCAGGGGTATCTAAT390.17695902717909162No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC370.16788420527247153No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTCTCTCATGGGGTATCTAATCCC340.1542719724125414No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTATCGTGGGCTCGGGGTATCTAA320.14519715050592133No Hit
TCTCAATCTAGAACAGGCTCCTCTATGCGTCAGGGGTATCTAATCCCAGT310.1406597395526113No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAA310.1406597395526113No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGCGGGGTATCTAATCCCAGTA310.1406597395526113No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACC310.1406597395526113No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAA290.1315849176459912No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC290.1315849176459912No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAG270.12251009573937112No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGTGGGGTATCTAATCCCAGTAA270.12251009573937112No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGTGGGGTATCTAATC260.11797268478606109No Hit
TCTCAATCTAGAACAGGCTCCTCTTATACACATGGGGTATCTAATCCCAG260.11797268478606109No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC240.108897862879441No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGT240.108897862879441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCT21700.0293.640563
CTCAATC21800.0292.970152
TCTAGAA21550.0292.946667
TCTCAAT22050.0292.324251
CAATCTA21850.0291.624734
AATCTAG21850.0290.27465
ATCTAGA21900.0289.611886
CTAGAAC21850.0288.249428
TAGAACA22600.0278.683629
TCAATAT150.0028308097196.666663
CTCAATA600.001785303773.749992
TATTTCT452.5465852E-1159.000004100-104
GTTTGGG501.8189894E-1259.00000430-34
CCTTAGA452.5465852E-1159.00000450-54
ACCGGGG257.787434E-659.00000430-34
GAATGGT257.787434E-659.00000485-89
CCAGGCA501.8189894E-1259.000004120-124
TAGGTTG501.8189894E-1259.00000420-24
GTTTACG650.059.000004110-114
GTGGGTC650.059.00000425-29
Produced by FastQC (version 0.11.5)