FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-96-5-1-3-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-96-5-1-3-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21567
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC690332.00723327305606No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC229710.650530903695461No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG15787.31673389901238No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGAT10384.812908610376965No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC6483.004590346362498No Hit
AATCCTATTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACC5162.3925441646960635No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC4782.21634905179209No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG3801.7619511290397367No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAAC3261.5115686001761952No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGA3171.469838178698938No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC2571.1916353688505588No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC2461.1406315203783557No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC2451.1359948068808827No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACC2391.108174525896045No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCG2331.080354244911207No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1520.7047804516158946No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA1430.6630500301386377No Hit
AATCCTATTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG1410.6537766031436918No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA1390.6445031761487457No Hit
AATCCTATTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACC1310.6074094681689618No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC1290.5981360411740159No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTA1140.528585338711921No Hit
AATCCTATTAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGT1100.510038484722029No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1060.4914916307321371No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT1010.4683080632447721No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACC820.3802105067927853No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC810.37557379329531226No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA800.3709370797978393No Hit
AATCCTATTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAAC780.3616636528028933No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAAC780.3616636528028933No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC760.3523902258079473No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA710.3292066583205824No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT620.28747623684332546No Hit
AATCCTATTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAA590.27356609635090645No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTAA580.2689293828534335No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT570.2642926693559605No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT510.23647238837112253No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC510.23647238837112253No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTAACCG510.23647238837112253No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTA490.22719896137617657No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT480.22256224787870357No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC480.22256224787870357No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC470.2179255343812306No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAAC460.2132888208837576No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTAA450.2086521073862846No Hit
AATCCTATTAGAACAGGCTCCTCTGGGTCTCGTGGGGTATCTAATCCCAG440.2040153938888116No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT410.19010525339639264No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAA410.19010525339639264No Hit
AATCCTATTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACC400.18546853989891965No Hit
AATCCTATTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATC400.18546853989891965No Hit
AATCCTATTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT390.18083182640144665No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA370.17155839940650067No Hit
AATCCTATTAGAACAGGCTCCTCTAGACATCGGGGTATCTAATCCCAGTA370.17155839940650067No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC360.16692168590902767No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC360.16692168590902767No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC350.16228497241155468No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTA340.1576482589140817No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG330.1530115454166087No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTAT310.14373811842166273No Hit
AATCCTATTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACC300.13910140492418974No Hit
AATCCTATTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC290.13446469142671674No Hit
AATCCTATTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCC270.12519126443177075No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCGGTCGGGGGTATCTAATCCCA270.12519126443177075No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC270.12519126443177075No Hit
AATCCTATTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT260.12055455093429776No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAA250.11591783743682478No Hit
AATCCTATTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTAACC250.11591783743682478No Hit
AATCCTATTAGAACAGGCTCCTCTAGACCATTTCGGGGGGTATCTAATCC240.11128112393935179No Hit
AATCCTATTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTAA240.11128112393935179No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTTCTGGGCTCGGAGGGGTATCTA240.11128112393935179No Hit
AATCCTATTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTA240.11128112393935179No Hit
AATCCTATTAGAACAGGCTCCTCTAGCATTGACAATCATTTCTGCGTTTC240.11128112393935179No Hit
AATCCTATTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGG220.1020076969444058No Hit
AATCCTATTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTAAC220.1020076969444058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATT108.407587E-4294.999973
CCGATTA108.407587E-4294.999974
CGATTAG108.407587E-4294.999975
AATCCTA21450.0292.937041
TCCTATT21400.0292.932223
CCTATTA21350.0292.92744
TATTAGA21300.0292.922526
CTATTAG21350.0292.23655
ATCCTAT21600.0290.90282
ATTAGAA21550.0290.208837
TTAGAAC21500.0289.511638
TAGAACA21950.0282.904339
AATCCGA150.0028304083196.666661
GATTAGA200.0066922093147.499986
ATCCGAT200.0066922093147.499982
TCAGAGA700.003288872863.21429295
GTTGAGG6650.059.000004105-109
GCTGTGG7000.059.00000460-64
TATTTCT1550.059.000004100-104
CAAGGTT1550.059.000004110-114
Produced by FastQC (version 0.11.5)