FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-95-5-2-6-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-95-5-2-6-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21336
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT671431.467941507311586No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT237911.150168728908886No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTT11885.568053993250844No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTG8914.176040494938133No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT6973.266779152605924No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT6102.8590176227971504No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGC4372.0481814773153353No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT3801.7810273715785525No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTT3301.546681664791901No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC2731.279527559055118No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT2561.1998500187476566No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2501.1717285339332584No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGC2471.1576677915260591No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGG2341.0967379077615298No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCT2130.9983127109111362No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTT2070.970191226096738No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2030.9514435695538057No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGG1800.843644544431946No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1730.8108361454818148No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCC1690.7920884889388826No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT1160.5436820397450318No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1100.5155605549306337No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1000.46869141357330335No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGT1000.46869141357330335No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTG920.43119610048743906No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTGG920.43119610048743906No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT920.43119610048743906No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT810.3796400449943757No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA710.33277090363704537No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGGC700.32808398950131235No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTTGTGGC690.3233970753655793No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTTG650.3046494188226472No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTT620.2905886764154481No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGGC600.281214848143982No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT590.276527934008249No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA580.2718410198725159No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGGC530.24840644919385074No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA530.24840644919385074No Hit
GAGACTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTTG530.24840644919385074No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTT500.23434570678665168No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTTG480.22497187851518563No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT480.22497187851518563No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTTGT470.22028496437945258No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGG470.22028496437945258No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT470.22028496437945258No Hit
GAGACTTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTTGT460.21559805024371953No Hit
GAGACTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTGGC450.2109111361079865No Hit
GAGACTTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTTGTGG450.2109111361079865No Hit
GAGACTTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT430.20153730783652046No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT400.18747656542932134No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGGC380.17810273715785527No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT370.17341582302212222No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT360.1687289088863892No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT350.16404199475065617No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGCAGCAGGGGTATCTAATCCCAGT350.16404199475065617No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT350.16404199475065617No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGTT340.15935508061492312No Hit
GAGACTTAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTTGT330.1546681664791901No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTTGTG330.1546681664791901No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA330.1546681664791901No Hit
GAGACTTAGAACAGGCTCCTCTAGTCAGCGTCAGGGGTATCTAATCCCAG310.14529433820772406No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTTG310.14529433820772406No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGGC300.140607424071991No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTTG290.13592050993625795No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTTG270.1265466816647919No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGTGG270.1265466816647919No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCT260.12185976752905887No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTTG260.12185976752905887No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTTGT260.12185976752905887No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTT250.11717285339332584No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAATCC240.11248593925759282No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTT230.10779902512185977No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCT230.10779902512185977No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT220.10311211098612673No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTGA108.406898E-4295.03
GAGACTT21100.0294.300931
ACTTAGA20900.0293.588534
CTTAGAA20800.0293.581735
GACTTAG20950.0292.887823
AGACTTA21100.0292.20382
TTAGAAC20950.0291.47976
GAACAGG20900.0290.765569
TAGAACA21900.0280.182657
AGAACAG21950.0278.872448
AGACTTG150.0028301778196.666672
GACTTAA150.0028301778196.666673
GAGACCT150.0028301778196.666671
AGACCTA200.006691664147.52
ACGTGAA200.006691664147.5295
GAGAGAA600.001784516573.75295
CGCTGCG150.00436961759.000004175-179
TAGCGGG150.00436961759.000004125-129
ACTGCCA600.059.00000435-39
GGGTTTG1050.059.00000425-29
Produced by FastQC (version 0.11.5)