FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-94-5-5-7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-94-5-5-7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21105
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC624229.575929874437335No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC19359.168443496801707No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT12145.752191423833215No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGC11115.264155413409145No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT6943.2883203032456767No Hit
CCACGTCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCC5842.767116796967543No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC4672.2127457948353473No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC4522.1416725894337834No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCC3211.5209665955934613No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATC2871.3598673300165838No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATC2841.345652688936271No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC2821.336176261549396No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA2491.179815209665956No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG2050.9713338071547027No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1990.9429045249940772No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1980.9381663113006397No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT1780.8434020374318882No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAG1750.8291873963515755No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG1720.8149727552712628No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1640.7770670457237622No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT1630.7723288320303245No Hit
CCACGTCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATC1380.6538734896943853No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG1070.5069888651978204No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCA970.4596067282634447No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC960.4548685145700071No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTC940.445392087183132No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC930.4406538734896943No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTC920.4359156597962568No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTCA870.41222459132906897No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCAT870.41222459132906897No Hit
CCACGTCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCC860.4074863776356314No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATC830.3932717365553186No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTCAT780.3695806680881308No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGTCAGGGGTATCTAATCCCAGT770.3648424543946932No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT720.3411513859275053No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTC690.32693674484719265No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTCAT620.2937692489931296No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCA620.2937692489931296No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTC590.2795546079128169No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCC590.2795546079128169No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTCA510.24164889836531628No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCC500.23691068467187867No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC500.23691068467187867No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTC500.23691068467187867No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCT470.222696043591566No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA440.20848140251125327No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCGCTTCGGAAGG440.20848140251125327No Hit
CCACGTCTAGAACAGGCTCCTCTAGCATCACGGGGGTATCTAATCCCAGT430.2037431888178157No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGCTCGGGGTATCTAATCCCAGTCA410.19426676143094054No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCT370.17531390665719024No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGG370.17531390665719024No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCC350.16583747927031509No Hit
CCACGTCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTC350.16583747927031509No Hit
CCACGTCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTCATC340.1610992655768775No Hit
CCACGTCTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTC330.15636105188343996No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCATCC330.15636105188343996No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCC330.15636105188343996No Hit
CCACGTCTAGAACAGGCTCCTCTAGCTTGCCAACAATCCTAGGGGTATCT330.15636105188343996No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTC310.1468846244965648No Hit
CCACGTCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAA310.1468846244965648No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCAT310.1468846244965648No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA280.13266998341625208No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTATCCGGGGTATCTAATCCCA280.13266998341625208No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTCGCGGGGTATCTAATCCCAGT270.1279317697228145No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCTGACGGGGTATCTAATCCCAGTC260.12319355602937693No Hit
CCACGTCTAGAACAGGCTCCTCTGGGTCTCGTGGGGTATCTAATCCCAGT260.12319355602937693No Hit
CCACGTCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT250.11845534233593934No Hit
CCACGTCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG250.11845534233593934No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCCGTGGGGTATCTAATCCCAGTC240.11371712864250177No Hit
CCACGTCTAGAACAGGCTCCTCTAGACATCTCGGCCAACGGGGTATCTAA240.11371712864250177No Hit
CCACGTCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTC230.1089789149490642No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCAT220.10424070125562664No Hit
CCACGTCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCC220.10424070125562664No Hit
CCACGTCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCC220.10424070125562664No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCCT108.406339E-4295.02
CACGTCT20700.0295.02
CCACGCC108.406339E-4295.01
CGTCTGA108.406339E-4295.04
CCACGTC20850.0294.292571
TCTAGAA20600.0292.851966
CGTCTAG20600.0292.851964
ACGTCTA20800.0291.45433
GTCTAGA20700.0291.43725
CTAGAAC20950.0290.775677
TAGAACA21250.0285.976478
AGAACAG21650.0280.692849
ACGTCTG150.0028299894196.666673
GTCTGAA150.0028299894196.666675
TCTGAAC150.0028299894196.666676
TCTAAAC200.0066912207147.56
CCACGAC455.6912075E-498.3333361
CGTTTGT600.059.00000460-64
TGTTGGT150.004369503459.00000495-99
TGGTCGG150.004369503459.000004210-214
Produced by FastQC (version 0.11.5)