FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-93-4-8-2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-93-4-8-2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21609
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT301113.934008977740756No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG285713.22134295895229No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGCTG21219.815354713313896No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT8854.095515757323338No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGTTG8804.072377250219816No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG7833.6234902124114954No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGTTG6002.7766208524226017No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT5852.707205331112037No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTGTT5202.406404738766255No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTGCT4522.09172104215836No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA3651.6891110185570828No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTGTT3411.5780461844601787No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT3011.3929381276320052No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTTGC3001.3883104262113009No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT2821.3050118006386229No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTGTT2621.212457772224536No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT2621.212457772224536No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT2481.1476699523346754No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTG2261.04586052107918No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG2231.031977416817067No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTG1720.7959646443611458No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA1310.606228886112268No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTATCGTGGGGTATCTAATCCCAGTG1160.5368133648017029No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1080.49979175343606835No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGTTG990.4581424406497293No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTGTG970.4488870378083206No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT950.43963163496691193No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTGTTG930.4303762321255033No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGTTG930.4303762321255033No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTGTGTTGC930.4303762321255033No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT890.4118654264426859No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTGT890.4118654264426859No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTGT870.40261002360127723No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGTTG850.3933546207598686No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTGTGT840.3887269193391642No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTGTG830.3840992179184599No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGCACATGGGGTATCTAATCCCAGT810.3748438150770512No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTGT690.31931139802859915No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTGT670.3100559951871905No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTG660.30542829376648617No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC640.29617289092507754No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC620.28691748808366885No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGTGT620.28691748808366885No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTCCGGGGTATCTAATCCCAGTGTGT610.2822897866629645No Hit
GAGTGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTGTGTTG590.2730343838215558No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT530.24526817529732983No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTAATATTATGGGGGTATCTAATCCC510.23601277245592112No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT510.23601277245592112No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTGTGT480.22212966819380814No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTGTG460.21287426535239948No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTGTGT460.21287426535239948No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT410.18973575824887778No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTGT400.18510805682817347No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCCGGGGTATCTAATCC390.1804803554074691No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTGT380.17585265398676478No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTGTG370.17122495256606043No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGCGGGGTATCTAATCCCAGTG360.16659725114535612No Hit
GAGTGGTAGAACAGGCTCCTCTATGTCTCGTGGGGTATCTAATCCCAGTG350.16196954972465177No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTGTT320.14808644546253877No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACACGGGTATCTAATCCCAGT320.14808644546253877No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTGCAGGGGTATCTAATCCCAGTG300.13883104262113008No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGCCGGGGGTATCTAATCCCAGTG300.13883104262113008No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTCGGGGTATCTAATCCCAGTGTGT300.13883104262113008No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTGTGT290.13420334120042574No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA280.12957563977972142No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT280.12957563977972142No Hit
GAGTGGTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTGT270.12494793835901709No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTCAGATGTGTATAAGGGGTATCTA270.12494793835901709No Hit
GAGTGGTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTGTGTTG270.12494793835901709No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTGT250.11569253551760841No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTTGC250.11569253551760841No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGGTATCTAATCCCAGTGTGTT240.11106483409690407No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGTGGGCTCGGAGATGGGGTATC240.11106483409690407No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTGTGT240.11106483409690407No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTGTG240.11106483409690407No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGCG230.10643713267619974No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTGTTG230.10643713267619974No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT220.1018094312554954No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGGT21350.0295.01
GAGGCGA108.407722E-4294.99997295
AGTGGTA21400.0294.310732
GTGGTAG21450.0292.937043
TGGTAGA21550.0291.577734
GGTAGAA21500.0290.883735
GAACAGG21500.0290.19779
GTAGAAC22050.0282.95926
AGAACAG22400.0278.537938
TAGAACA22550.0276.685157
GGTAGAC150.0028304534196.666675
GCCCTCG303.3140896E-759.00000425-29
TCCTTGG303.3140896E-759.00000435-39
ATCTCCT303.3140896E-759.00000430-34
TCTAGGC600.059.00000420-24
TCGATCT303.3140896E-759.00000425-29
CTCCTTG303.3140896E-759.00000430-34
TTTCTCA150.004369783759.00000490-94
GTACTCT303.3140896E-759.00000475-79
CCTCGAT303.3140896E-759.00000425-29
Produced by FastQC (version 0.11.5)