FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-92-5-2-6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-92-5-2-6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21074
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC312314.81920850336908No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG255612.128689380279017No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCC15817.502135332637374No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC10665.058365758754864No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG7983.786656543608238No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTG7853.7249691563063494No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGT6363.017936794153934No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGC4382.0783904337097847No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC3911.8553668026952643No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA3881.8411312517794438No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA3571.6940305589826326No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC3121.480497295245326No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTG2761.3096706842554806No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGG2481.17680554237449No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTT2251.0676663186865332No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGT2211.0486855841321059No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGT2131.0107241150232513No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATC2030.9632722786371832No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGT1980.9395463604441492No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT1960.9300559931669357No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG1840.8731137895036538No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1490.7070323621524153No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGT1440.6833064439593812No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCA1430.6785612603207745No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGTGGGCTCGGAGGGGTATCT1270.6026383221030653No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA1150.5456961184397836No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT1130.53620575116257No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG1090.5172250166081427No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTG1060.5029894656923223No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1040.49349909841510864No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1030.4887539147765019No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC1010.4792635474992883No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA980.46502799658346783No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC960.45553762930625413No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTTG920.4365568947518269No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT900.42706652747461327No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGT850.4033406092815792No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATC830.3938502420043656No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT810.38435987472715194No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTG770.3653791401727247No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGCTCGGGGGTATCTAATCCCAGT760.3606339565341179No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG750.355888772895511No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGT750.355888772895511No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCC740.35114358925690425No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGACATCGGGGTATCTAATCCCAGTT740.35114358925690425No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG710.3369080383410838No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTT710.3369080383410838No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC670.31792730378665657No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAG650.3084369365094429No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGT590.27996583467780206No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA580.27522065103919524No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTTG560.2657302837619816No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTG530.25149473284616114No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGT490.23251399829173391No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTTG480.22776881465312707No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTA460.21827844737591345No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTTG450.21353326373730663No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT440.20878808009869984No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACC440.20878808009869984No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC430.204042896460093No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTTAGGCATCTCGGGGTATCTAAT410.19455252918287938No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTTG410.19455252918287938No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTTGT390.18506216190566574No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACCGGGGTATCTAATCCCAGTTG380.18031697826705895No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTG380.18031697826705895No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTTG370.17557179462845213No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTCGTGGGGTATCTAATCCCAGTT370.17557179462845213No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTTGT360.1708266109898453No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGG360.1708266109898453No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC360.1708266109898453No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGCCCTTTATAGGGGTATCTAATCCC350.1660814273512385No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC330.15659106007402485No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCGTGGGCTCGGGGTATCTAAT320.15184587643541805No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCA320.15184587643541805No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTTGTG300.1423555091582044No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGCTCGGGGTATCTAATCCCAG300.1423555091582044No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG300.1423555091582044No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGATCTGCAGCACGCGGAGGGGGTAT290.13761032551959762No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGT270.12811995824238398No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGAGGGGTA250.11862959096517035No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCTGAGGGGTATC250.11862959096517035No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTTCGTGGGCTCGGAGATGGGGTA240.11388440732656353No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCA240.11388440732656353No Hit
TTCTCAGCTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTC220.10439404004934992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGCTA20800.0295.04
GCTAGAA20700.0294.287457
TTCTCAG21050.0293.598571
CTCAGCT20950.0293.59193
TCTCAGC20950.0293.59192
CAGCTAG20900.0292.882785
CTAGAAC20800.0290.74528
AGCTAGA21100.0290.106636
TAGAACA21150.0285.23649
ATGAGGA150.0028299422196.66666295
GCAGGAG200.0066911085147.5295
CCAACAA650.00245093968.07693295
CTCTGGC452.5465852E-1159.00000480-84
TCTAGGA1550.059.00000420-24
CTCCTCT20750.059.00000415-19
GCGGTTT452.5465852E-1159.00000485-89
GGCGAGC3600.059.00000480-84
GCGGATA1550.059.00000485-89
AGGGATG452.5465852E-1159.00000425-29
CTGGCGG1550.059.00000480-84
Produced by FastQC (version 0.11.5)