FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-91-5-2-6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-91-5-2-6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20788
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT576527.732345583990764No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT18969.120646526842409No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTT12666.090051953049837No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTG11105.339619010967866No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT7053.3913796421012123No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGC6202.982489898018087No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT5382.588031556667308No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT3011.447950740812007No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGG2901.395035597460073No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTT2841.3661727919953819No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGG2661.2795843756013086No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCT2631.265152972868963No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT2541.2218587646719261No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC2491.1978064267846835No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTT2081.0005772561092938No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGC2070.9957667885318452No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1870.8995574369828747No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT1840.8851260342505292No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTTG1700.8177794881662497No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTG1490.7167596690398307No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCC1410.6782759284202424No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT1350.6494131229555513No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTTG1170.5628247065614778No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT1020.4906676928997498No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT1010.4858572253223013No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT960.4618048874350587No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGG900.43294208197036754No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA860.41370021166057347No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTGG830.3992688089282278No Hit
GAGTGGTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCAC810.38964787377333077No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTT780.37521647104098516No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAGT750.3607850683086396No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGT720.346353665576294No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCC700.33673273042139695No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT640.3078699249567058No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTTG620.29824898980180875No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGGGG620.29824898980180875No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA590.28381758706946314No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTTGTG580.2790071194920146No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA550.264575716759669No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTT510.2453338464498749No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGCTCGGGGTATCTAATCCCAGTT490.23571291129497787No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT490.23571291129497787No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTATAGGGGTATCTAATCCCAGTT490.23571291129497787No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGTG480.23090244371752935No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGCGGGGTATCTAATCCCAGTTGT470.2260919761400808No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGGTATCTAAT470.2260919761400808No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTTGTG450.21647104098518377No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTTGTG440.21166057340773525No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGGGGGTATCTAATCCCAGTTGT440.21166057340773525No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT420.20203963825283816No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT420.20203963825283816No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTTG410.19722917067538964No Hit
GAGTGGTAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT410.19722917067538964No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGGC410.19722917067538964No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCT390.18760823552049258No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGGC390.18760823552049258No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTTGTG380.18279776794304406No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT380.18279776794304406No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCACTCAGGGGTATCTAATCCCAGTT370.1779873003655955No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT370.1779873003655955No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTTG360.173176832788147No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT350.16836636521069848No Hit
GAGTGGTAGAACAGGCTCCTCTACTGGGGGTATCTAATCCCAGTTGTGGC340.16355589763324996No Hit
GAGTGGTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTGGC340.16355589763324996No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGTGGGCTCGGAGATGGGGTATC330.15874543005580144No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC330.15874543005580144No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCCGGGGTATCTAATCC330.15874543005580144No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT290.1395035597460073No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT290.1395035597460073No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGCGTCAGGGGTATCTAATCCCAGT280.13469309216855876No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTT280.13469309216855876No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGTCGGGGGTATCTAATCCCAGT260.12507215701366173No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTT250.1202616894362132No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTTGTG240.11545122185876468No Hit
GAGTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT230.11064075428131614No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTTGTG220.10583028670386763No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTTGTG220.10583028670386763No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTT108.4053323E-4294.999971
TGTTAGA108.4053323E-4294.999974
GTTAGAC108.4053323E-4294.999975
GGTAGAA20450.0294.278725
AGTGGTA20550.0293.564482
TGGTAGA20500.0293.560974
GAGTGGT20600.0292.851961
GAACAGG20500.0291.402449
GTGGTAG20800.0289.326933
GTAGAAC20900.0286.53116
AGAACAG21200.0281.780648
TAGAACA21550.0277.20427
GTGTTAG150.0028296516196.666663
AGAACGG150.0028296516196.66666295
TAGACCA150.0028296516196.666667
CCATGAA200.006690424147.49998295
GCAGCCA200.006690424147.49998295
AGTGTTA200.006690424147.499982
CACCACG200.006690424147.49998295
GTGACGA200.006690424147.49998295
Produced by FastQC (version 0.11.5)