FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-90-4-1-3-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-90-4-1-3-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21001
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC353916.851578496262082No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGT310114.765963525546402No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTAACCGATC16818.004380743774107No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCC11035.252130850911861No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTAACCG7653.6426836817294417No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGT5612.67130136660159No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTAACC5532.6332079424789296No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTAACCGA4091.9475263082710348No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTAACCGAT4041.9237179181943718No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTAACCG3331.585638779105757No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCC3221.5332603209370983No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCC3191.5189752868911004No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGT2741.3046997762011334No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAG2591.2332746059711444No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTAACC2411.1475644016951574No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCC2371.128517689633827No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTA2040.971382315127851No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTA2000.9523356030665207No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAG1990.9475739250511881No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTAAC1410.6713966001618971No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTCAGGGGTATCTAATCCCAGTAA1390.6618732441312318No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTA1350.6428265320699014No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGT1300.6190181419932385No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCC1280.6094947859625732No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCC1240.5904480739012428No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTAACC1210.576163039855245No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCC1190.5666396838245799No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAG1010.48092947954859294No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTAACC980.46664444550259515No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTAA960.45712108947192986No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTAACCG960.45712108947192986No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTA940.4475977334412647No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTAA930.4428360554259321No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGT840.39998095328793865No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTA830.39521927527260603No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCA790.3761725632112757No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGT790.3761725632112757No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGT720.3428408171039474No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCACACCTCAAGGTACCCTCCCTGCA680.323794105042617No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTAACCG660.3142707490119518No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTAACCG660.3142707490119518No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCC640.3047473929812866No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCA630.299985714965954No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCC580.276177324889291No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTAAC580.276177324889291No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTAACCG580.276177324889291No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCC550.2618922908432932No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTCGGGGGTATCTAATCCCAGTAAC530.25236893481262795No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAG510.24284557878196275No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTAACC500.23808390076663016No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTA480.22856054473596493No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTA480.22856054473596493No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTAAC460.21903718870529976No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAAT450.21427551068996714No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGCCGGGGTATCTAATC440.20951383267463455No Hit
GCGAAGTTAGAACAGGCTCCTCTTATAGGGGTATCTAATCCCAGTAACCG420.19999047664396932No Hit
GCGAAGTTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTAAC420.19999047664396932No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCC410.19522879862863673No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGGGGGTATCTAATCCCAGTAAC370.17618208656730633No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTAACCG360.1714204085519737No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCCA360.1714204085519737No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGTA360.1714204085519737No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGACTACTCGGGGTATCTAATCCCAG330.1571353745059759No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTTGGTCGGGGTATCTAATCCCAGT320.1523736964906433No Hit
GCGAAGTTAGAACAGGCTCCTCTATACACATGGGGTATCTAATCCCAGTA300.1428503404599781No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGG290.1380886624446455No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACGTCGGTCAGGGGTATCTAATCCC290.1380886624446455No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGCTCGGGGGTATCTAATCCCAGTA270.12856530641398028No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTAA270.12856530641398028No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTA270.12856530641398028No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATTGGGGGGTATCTAATCCCAGTAA260.12380362839864768No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCA260.12380362839864768No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCC260.12380362839864768No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTGTGATTTGAGCCGATTGTGGAACG250.11904195038331508No Hit
GCGAAGTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCAGTA250.11904195038331508No Hit
GCGAAGTTAGAACAGGCTCCTCTAGATCTCGGGGTATCTAATCCCAGTAA240.11428027236798247No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCT230.10951859435264988No Hit
GCGAAGTTAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTAA230.10951859435264988No Hit
GCGAAGTTAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCC220.10475691633731728No Hit
GCGAAGTTAGAACAGGCTCCTCTAGTCGCGGCACGCAGGGGTATCTAATC220.10475691633731728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGTT20700.0294.287452
GCGAAGT20950.0293.59191
GAAGTTA20700.0293.57493
AAGTTAG20700.0292.862344
AGTTAGA20800.0291.45435
TTAGAAC20700.0291.43727
GTTAGAA20700.0291.43726
TAGAACA22050.0272.925178
AGAACAG22050.0272.256239
CGAAGTA304.562844E-7196.666662
GCGAAAG150.0028298942196.666661
CGAAAGT150.0028298942196.666662
GAAGTAG505.8027435E-6118.03
AAGTAGA703.086235E-584.285714
AGTAGAA951.4012096E-462.105265
TGTTGAG4050.059.000004105-109
GTTGAGG4050.059.000004105-109
GAGCGGT257.784973E-659.00000485-89
GGTCCTT4050.059.00000445-49
GGACTAC1250.059.00000425-29
Produced by FastQC (version 0.11.5)