FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-9-4-2-6-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-9-4-2-6-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11592
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT209018.02967563837129No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTT10739.256383712905453No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTG8867.6432022084196No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT7346.331953071083506No Hit
GAGACTTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGC4764.1062801932367154No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTT3983.433402346445825No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGG2452.1135265700483092No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT2432.096273291925466No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2342.018633540372671No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGC2322.001380262249828No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT2121.828847481021394No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTT2111.8202208419599724No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCT1741.5010351966873705No Hit
GAGACTTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA1731.492408557625949No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGG1661.4320220841959972No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCC1391.1991028295376123No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT1221.0524499654934438No Hit
GAGACTTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTTGTGG1080.9316770186335404No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGT1060.914423740510697No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT980.8454106280193237No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT980.8454106280193237No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT730.6297446514837819No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTTGTGGC680.5866114561766735No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTG670.5779848171152518No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT660.5693581780538302No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTTGT650.5607315389924086No Hit
GAGACTTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTTGTGGC640.5521048999309869No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTT590.5089717046238785No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA580.500345065562457No Hit
GAGACTTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTTG550.47446514837819187No Hit
GAGACTTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGGC540.4658385093167702No Hit
GAGACTTAGAACAGGCTCCTCTAGCTCGGGGTATCTAATCCCAGTTGTGG510.4399585921325052No Hit
GAGACTTAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTGGC510.4399585921325052No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTCGTGGGGTATCTAATCCCAGTTG500.4313319530710835No Hit
GAGACTTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGGC480.4140786749482402No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGG470.4054520358868185No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTT470.4054520358868185No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGTTG460.3968253968253968No Hit
GAGACTTAGAACAGGCTCCTCTAGACATTGGGGTATCTAATCCCAGTTGT450.38819875776397517No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGTCGGTCAGGGGTATCTAATCCCA440.3795721187025535No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGCAGCAGGGGTATCTAATCCCAGT430.3709454796411318No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTTG430.3709454796411318No Hit
GAGACTTAGAACAGGCTCCTCTAGGACTACGGGGTATCTAATCCCAGTTG410.35369220151828845No Hit
GAGACTTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTTGT370.3191856452726018No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCGGGGTATCTAATCCCAGTTGTG320.27605244996549344No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC310.26742581090407175No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTTG300.2587991718426501No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT300.2587991718426501No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT300.2587991718426501No Hit
GAGACTTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGGC300.2587991718426501No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT280.24154589371980675No Hit
GAGACTTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTTGTGG280.24154589371980675No Hit
GAGACTTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGGC270.2329192546583851No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT250.21566597653554176No Hit
GAGACTTAGAACAGGCTCCTCTAGTCTGACAGGGGTATCTAATCCCAGTT250.21566597653554176No Hit
GAGACTTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT250.21566597653554176No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCCTGGGGTATCTAATCCCAGTTGT250.21566597653554176No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTTG230.1984126984126984No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACCCGGGGTATCTAATCCCAGTT230.1984126984126984No Hit
GAGACTTAGAACAGGCTCCTCTATACGGGGTATCTAATCCCAGTTGTGGC220.18978605935127674No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTTGGGGTATCTAATCCCAGTTGTG220.18978605935127674No Hit
GAGACTTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA210.18115942028985507No Hit
GAGACTTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAG210.18115942028985507No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTT200.1725327812284334No Hit
GAGACTTAGAACAGGCTCCTCTAGTCAGCGTCAGGGGTATCTAATCCCAG200.1725327812284334No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGCGGGCTTGGGGTATCTAATC180.15527950310559005No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGTTG170.14665286404416839No Hit
GAGACTTAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTTGTG160.13802622498274672No Hit
GAGACTTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT160.13802622498274672No Hit
GAGACTTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTT160.13802622498274672No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAGTT150.12939958592132506No Hit
GAGACTTAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTTGTGG120.10351966873706005No Hit
GAGACTTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT120.10351966873706005No Hit
GAGACTTAGAACAGGCTCCTCTAGGTCGTGGGGTATCTAATCCCAGTTGT120.10351966873706005No Hit
GAGACTTAGAACAGGCTCCTCTAGTCAGGAAAGTGATAAATTTTTCTCGT120.10351966873706005No Hit
GAGACTTAGAACAGGCTCCTCTAGAGACGGGGTATCTAATCCCAGTTGTG120.10351966873706005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAA11300.0295.05
ACTTAGA11300.0295.04
GACTTAG11300.0295.03
GACTTAA157.056615E-6294.999973
AGACTTA11500.0293.71742
GAGACTT11500.0293.71741
TTAGAAC11350.0292.400886
GAACAGG11450.0291.135389
TAGAACA11950.0278.953987
AGAACAG12200.0273.23778
CTTAAAC200.0066521815147.55
ACTTAAA301.1200718E-4147.499984
TTTTCTA452.5465852E-1159.000004100-104
GACGGGG452.5465852E-1159.00000420-24
GAGTTTT3500.059.00000450-54
CTCTAGG6950.059.00000415-19
AGGTGGG1650.059.00000420-24
GCTTGTA1650.059.00000465-69
TACTGGG452.5465852E-1159.00000425-29
CAGGCTC11500.059.00000410-14
Produced by FastQC (version 0.11.5)