FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-89-5-2-6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-89-5-2-6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20505
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT654831.933674713484518No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT225410.992440868080957No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTT14407.022677395757132No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTTGTGGCCTG9194.4818336990977805No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT7053.4381858083394294No Hit
TGCTTATAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTTGTGGC6483.1602048280907096No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT5472.667642038527189No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT3751.8288222384784198No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTTGTGGCCT2431.1850768105340161No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT2341.141185076810534No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT2341.141185076810534No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTTG2111.0290173128505242No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTTGTGG2111.0290173128505242No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT1460.7120214581809314No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTTGTG1380.6730065837600585No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT1370.6681297244574494No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC1270.6193611314313582No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTTGTGG1230.5998536942209217No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTT1140.5559619604974396No Hit
TGCTTATAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTTGTGGCC1080.5267008046817849No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTT1060.5169470860765667No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT1050.5120702267739575No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTTGTGG1040.5071933674713485No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTTGTG990.4828090709583029No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT980.4779322116556938No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTTGTGGC960.46817849305047554No Hit
TGCTTATAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA940.4584247744452572No Hit
TGCTTATAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCAGT930.4535479151426481No Hit
TGCTTATAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTTG860.4194099000243843No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT720.3511338697878566No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT690.33650329188002925No Hit
TGCTTATAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTTGTGGC630.30724213606437456No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTAGTGGGGTATCTAATCCCAGTT600.2926115581565472No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGGGGTATCTAATCCCAGTTGT600.2926115581565472No Hit
TGCTTATAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAA590.2877346988539381No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTTGT510.24871982443306512No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTTGTGGC500.243842965130456No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTT490.2389661058278469No Hit
TGCTTATAGAACAGGCTCCTCTAGCTACAGGGGTATCTAATCCCAGTTGT470.2292123872226286No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCT470.2292123872226286No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTTG460.22433552792001948No Hit
TGCTTATAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCC450.21945866861741037No Hit
TGCTTATAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTTGTGGC440.21458180931480125No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTTG440.21458180931480125No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTTGTGGCC430.20970495001219214No Hit
TGCTTATAGAACAGGCTCCTCTAGAAGGCTATAAAGCACCGCCAAGTCCT410.1999512314069739No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT390.19019751280175565No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTA370.18044379419653742No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTT370.18044379419653742No Hit
TGCTTATAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTTGT370.18044379419653742No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGTTG360.1755669348939283No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC360.1755669348939283No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA360.1755669348939283No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT330.16093635698610095No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT330.16093635698610095No Hit
TGCTTATAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAAT320.15605949768349184No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAGTT320.15605949768349184No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGAACTGCCAGGTCCT270.13167520117044623No Hit
TGCTTATAGAACAGGCTCCTCTAGCGGGGTATCTAATCCCAGTTGTGGCC260.12679834186783712No Hit
TGCTTATAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCT250.121921482565228No Hit
TGCTTATAGAACAGGCTCCTCTAGGGCTCGGGGGTATCTAATCCCAGTTG240.11704462326261889No Hit
TGCTTATAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGT240.11704462326261889No Hit
TGCTTATAGAACAGGCTCCTCTAGACTACTCGGGGTATCTAATCCCAGTT240.11704462326261889No Hit
TGCTTATAGAACAGGCTCCTCTAGGTCTCGTGGCTCGGGGTATCTAATCC230.11216776396000974No Hit
TGCTTATAGAACAGGCTCCTCTAGAGGGATATAAAGCATGGCCAAGTCCT230.11216776396000974No Hit
TGCTTATAGAACAGGCTCCTCTAGTAGGGGTATCTAATCCCAGTTGTGGC220.10729090465740063No Hit
TGCTTATAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTTGTGGC220.10729090465740063No Hit
TGCTTATAGAACAGGCTCCTCTAGGTTGGGGGTATCTAATCCCAGTTGTG220.10729090465740063No Hit
TGCTTATAGAACAGGCTCCTCTAGATCGGGGTATCTAATCCCAGTTGTGG220.10729090465740063No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAGG108.404591E-4295.09
TGCTTCT108.404591E-4295.01
TTCTAGA108.404591E-4295.04
GCTTCTA108.404591E-4295.02
TGCTTAT20000.0294.26251
GCTTATA20000.0292.78752
CTTATAG19850.0292.770783
TTATAGA19900.0291.293984
GAACAGG19800.0291.275249
TATAGAA20200.0285.507425
ATAGAAC20200.0284.777226
AGAACAG20300.0284.100988
TAGAACA20700.0278.61117
GAACGGC202.2496904E-5221.259
AGAACGG202.2496904E-5221.258
TGCTTAA150.0028294027196.666671
CTTCTAG150.0028294027196.666673
TTATAAA200.0066898363147.54
TAGAACG403.5610714E-4110.6257
TTGTAGA401.2504523E-473.754
Produced by FastQC (version 0.11.5)