FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-88-6-4-8-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-88-6-4-8-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20153
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT1096954.42862104897534No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT14086.9865528705403666No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT9814.867761623579616No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT5862.907755669131147No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT4992.4760581551133827No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT4412.188259812434873No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGGTATCTAATCCCAGTGTCAAT4102.0344365603136008No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCT4001.9848161564035132No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTAAAGCACCGCCAAGTCCT3921.9451198332754427No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTCAATCAC3251.6126631270778544No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTT2991.483650076911626No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTGTCAA2491.2355480573611868No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTGTCAAT2271.1263831687589936No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCAGGGGTATCTAAT1960.9725599166377213No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTG1750.868357068426537No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTCAA1660.8236987049074579No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTCAA1650.8187366645164491No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGGTATCTAATCCCAGTGTCAATC1390.6897236143502208No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTCAA1250.6202550488760978No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTCAAT1190.5904828065300451No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTCAATC750.37215302932565875No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTG650.3225326254155709No Hit
GAGTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT510.2530640599414479No Hit
GAGTGGTAGACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT380.18855753485833376No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT240.11908896938421078No Hit
GAGTGGTAAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT240.11908896938421078No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCT220.10916488860219323No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGGA108.4035244E-4295.01
AGTGGTA20050.0295.02
GTACAGG108.4035244E-4295.06
GTGGTAC108.4035244E-4295.03
TGGTAAA157.1354825E-6294.999974
GAGTGGT20100.0294.266171
TGGTAGA19800.0293.51014
GGTAGAA19800.0293.51015
GTGGTAG19850.0292.770783
GAACAGG19750.0292.012669
GTAGAAC20300.0284.82766
TAGAACA20350.0284.127787
AGAACAG20700.0278.61118
GTGGTAA202.2491198E-5221.253
TGGTATA150.0028290446196.666664
TGGTACA150.0028290446196.666664
AGTGAAG150.0028290446196.66666295
GGTACAG150.0028290446196.666665
TACAGGC200.006688992147.57
AGTGGAG200.006688992147.52
Produced by FastQC (version 0.11.5)