FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-87-5-5-7-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-87-5-5-7-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20155
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT615430.533366410320017No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCTT18589.218556189531133No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTCA13186.539320267923592No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCTG8724.326469858595882No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCTT5412.6841974696105186No Hit
ATCTGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCAG4882.4212354254527413No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCTT4472.217811957330687No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAGC4372.168196477300918No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCCA3941.95484991317291No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTCA2681.3296948647978168No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCTT2611.2949640287769784No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTCA2491.2354254527412554No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATCCA2371.1758868767055322No Hit
ATCTGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAAT2311.1461175886876707No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCAG2231.106425204663855No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCTT1950.967501860580501No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCAT1710.8484247085090548No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCTT1650.8186554204911933No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGCT1540.7640783924584471No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATCC1410.6995782684197469No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGTC1110.5507318283304391No Hit
ATCTGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTCA1040.5160009923096006No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATCCA970.48127015628876213No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCTT880.4366162242619698No Hit
ATCTGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTCA820.4068469362441081No Hit
ATCTGTAGAACAGGCTCCTCTAGTGTTAGGGGGGTATCTAATCCCAGTCA820.4068469362441081No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCTT810.40188538824113124No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCAG740.3671545522202927No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGTC720.3572314562143389No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCATC680.33738526420243115No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCAT670.3324237161994542No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCTT600.2976928801786157No Hit
ATCTGTAGAACAGGCTCCTCTAGGGTCAGGGGTATCTAATCCCAGTCATC560.27784668816670804No Hit
ATCTGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCCAG540.2679235921607541No Hit
ATCTGTAGAACAGGCTCCTCTAGTGGCTCGGGGTATCTAATCCCAGTCAT540.2679235921607541No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTAATC530.26296204415777724No Hit
ATCTGTAGAACAGGCTCCTCTAGGTTCGGGGTATCTAATCCCAGTCATCC520.2580004961548003No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTCCTT520.2580004961548003No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGGG520.2580004961548003No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCAT510.2530389481518234No Hit
ATCTGTAGAACAGGCTCCTCTAGATCTCGTGGGGTATCTAATCCCAGTCA510.2530389481518234No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCCAGT490.2431158521458695No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGTC490.2431158521458695No Hit
ATCTGTAGAACAGGCTCCTCTAGCTTGGGGTATCTAATCCCAGTCATCCA480.23815430414289257No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCATCCAG480.23815430414289257No Hit
ATCTGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCCA450.2232696601339618No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTCCTT440.2183081121309849No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCCAG440.2183081121309849No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTCAT420.208385016125031No Hit
ATCTGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCAT420.208385016125031No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTTGGGGTATCTAATCCCAGTC400.19846192011907715No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATCCA370.18357727611014635No Hit
ATCTGTAGAACAGGCTCCTCTAGCTACTGGGGTATCTAATCCCAGTCATC370.18357727611014635No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCCTTT360.17861572810716944No Hit
ATCTGTAGAACAGGCTCCTCTAGCATATTGGGGTATCTAATCCCAGTCAT350.1736541801041925No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGGGGGGTATCTAATCCCAGTCATCC350.1736541801041925No Hit
ATCTGTAGAACAGGCTCCTCTAGCACGTCAGGGGTATCTAATCCCAGTCA340.16869263210121557No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCTTT340.16869263210121557No Hit
ATCTGTAGAACAGGCTCCTCTAGGGGGGATGGAAACACCATACCACCAAG310.15380798809228477No Hit
ATCTGTAGAACAGGCTCCTCTAGCGTAATGTGTATAGAGGGGTATCTAAT310.15380798809228477No Hit
ATCTGTAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTCATC290.14388489208633093No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGGGGTATCTAATCCCAGTCATCC290.14388489208633093No Hit
ATCTGTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTCATCC290.14388489208633093No Hit
ATCTGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTCCTT290.14388489208633093No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGGTTTGAGGCACCGCCAAGTCCTT290.14388489208633093No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCCA280.13892334408335402No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCATC280.13892334408335402No Hit
ATCTGTAGAACAGGCTCCTCTAGGTGGATCTAAAGCACCGCCAAGTCCTT280.13892334408335402No Hit
ATCTGTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCAGTC280.13892334408335402No Hit
ATCTGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCCAG260.12900024807740015No Hit
ATCTGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATCCAG240.11907715207144628No Hit
ATCTGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTGGGGTATCTA240.11907715207144628No Hit
ATCTGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGAGGGGTATCTAA230.11411560406846936No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTGG108.4035244E-4295.0295
TCTGTAG19900.0295.02
ATCTGTA20050.0294.264341
GAACAGG19650.0294.249368
CTGTAGA20000.0293.5253
AACAGGC19600.0293.49499
GTAGAAC20350.0285.57745
TGTAGAA20550.0284.951354
TAGAACA20550.0282.08036
AGAACAG20600.0281.395637
TCTGTAA150.0028290446196.666662
GTAGAAA455.687435E-498.333335
TTCTGGC7100.059.00000475-79
AGTGTTC7500.059.00000470-74
GGCTAAG7500.059.000004115-119
CCAGGTC6700.059.00000440-44
TAGCGGG201.839355E-459.0125-129
TGGTCGA257.7826935E-659.0210-214
TTGTTGA7300.059.090-94
GGGTTTG650.059.025-29
Produced by FastQC (version 0.11.5)