FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-86-4-5-7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-86-4-5-7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20296
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAGTC285914.086519511233742No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCT252112.421166732361057No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTCATCCAGCT19379.543752463539613No Hit
GAGTGGTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTCATCCA9874.863027197477336No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAGTC7913.897319668900276No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTCCT7263.5770595191170673No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAGTC6583.242018131651557No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTCATCC5012.4684666929444226No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTCATCC4792.360070949940875No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTCATCCA3501.724477729601892No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCTAA3081.517540402049665No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCAGT2661.310603074497438No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTCATCCAGC2351.1578636184469846No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGTCAGGGGTATCTAATCCCAGTCA2341.1529365392195507No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCAGT2331.1480094599921167No Hit
GAGTGGTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTCATCCA1740.857311785573512No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTCCT1730.8523847063460781No Hit
GAGTGGTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAGTC1620.7981868348443043No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTCATCCAG1610.7932597556168702No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTCAT1600.7883326763894364No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTCATC1560.7686243594797004No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTCCT1540.7587702010248325No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTCATCC1470.7242806464327947No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGTGGGGTATCTAATCCCAGTCA1390.6848640126133229No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGTGGGGTATCTAATCCCAGTCA1390.6848640126133229No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAGTC1350.6651556957035869No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGTATCTAATCCCAGTCATC1220.6011036657469452No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTCATCCA1130.5567599527000394No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACCGGGGTATCTAATCCCAGTCAT1030.5074891604256997No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCAGT1010.49763500197083166No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGTCGGGGTATCTAATCCCAGTCAT1000.4927079227433977No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAGTC990.48778084351596374No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTACAGGGGTATCTAATCCCAGTCAT970.47792668506109576No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTCATCC960.4729996058336618No Hit
GAGTGGTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGTCA890.438510051241624No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGGGGGGTATCTAATCCCAGTC860.423728813559322No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGTCA830.4089475758770201No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTCCT800.3941663381947182No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATTGGGGTATCTAATCCC750.3695309420575483No Hit
GAGTGGTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGTCA740.3646038628301143No Hit
GAGTGGTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTCATCCA690.33996846669294445No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCAGGGGTATCTAATCCCAGTCATC670.33011430823807647No Hit
GAGTGGTAGAACAGGCTCCTCTAGATCTCGGGGTATCTAATCCCAGTCAT660.3251872290106425No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTCGTGGGGTATCTAATCCCAGTCA660.3251872290106425No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCCCA650.32026014978320855No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTCATCCA650.32026014978320855No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTCCT650.32026014978320855No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTCCT620.30547891210090655No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAGTC620.30547891210090655No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAGGG580.2857705951911707No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCCAG570.2808435159637367No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTTCGGGGTATCTAATCCCAGTCAT540.26606227828143475No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAATC530.2611351990540008No Hit
GAGTGGTAGAACAGGCTCCTCTAGGCTCGGGGTATCTAATCCCAGTCATC530.2611351990540008No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTGGGGGTATCTAATCCCAGTCA480.2364998029168309No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGTGGGGTATCTAATCCCAGTCAT460.22664564446196295No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGTGCGGGGTATCTAATCCCAGT440.21679148600709497No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTCATCCA440.21679148600709497No Hit
GAGTGGTAGAACAGGCTCCTCTAGCAGGGGTATCTAATCCCAGTCATCCA410.20201024832479306No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGGCGGGGTATCTAATCCCA410.20201024832479306No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCCGTGGGGGTATCTAATCCCAGTC400.1970831690973591No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGTTGGGGTATCTAATCCCAGTCA400.1970831690973591No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTCGTGAGGTATCTAATCCCAGTC400.1970831690973591No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGTGGGGGTATCTAATCCCAGTCAT380.18722901064249114No Hit
GAGTGGTAGAACAGGCTCCTCTAGTGGCTCGGGGTATCTAATCCCAGTCA360.17737485218762317No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTCCT360.17737485218762317No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCGGGGGGTATCTAATCCCAGTCAT330.16259361450532125No Hit
GAGTGGTAGAACAGGCTCCTCTAGCGCTCTTCCGATCTAGGGGTATCTAA310.15273945605045328No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTCTGGGGGTATCTAATCCCAGTCAT300.14781237682301931No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTTGCGGGGGTATCTAATCCCAGTCA300.14781237682301931No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCTCCAACAGCGGGGTATCTAATCCC280.13795821836815136No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTCCT280.13795821836815136No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGGGGGTATCTAATCCCAGTCATC280.13795821836815136No Hit
GAGTGGTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTCCT270.13303113914071737No Hit
GAGTGGTAGAACAGGCTCCTCTAGGGACTACCCGGGGTATCTAATCCCAG270.13303113914071737No Hit
GAGTGGTAGAACAGGCTCCTCTAGTCGGGCTCGGGGTATCTAATCCCAGT270.13303113914071737No Hit
GAGTGGTAGAACAGGCTCCTCTAGGTGGCGGGGGTATCTAATCCCAGTCA220.10839574300354748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA19750.0295.04
CGGACAA108.4038323E-4295.0295
GAGTGGT20050.0293.52871
GTGGTAG19850.0293.513853
GGTAGAA19800.0292.765145
AGTGGTA20050.0291.321722
GAACAGG19950.0289.08529
GTAGAAC20550.0281.362556
TAGAACA20900.0276.650737
AGAACAG20950.0275.990458
GGTGAAC150.002829148196.666675
TGGTGAA150.002829148196.666674
AGTGGTG200.0066892356147.52
GTGGTGA200.0066892356147.53
CAAGCGA200.0066892356147.5295
GAGTGTA352.0926683E-4126.4285661
AGTGTAG550.001262463980.4545442
GCCCTCG501.8189894E-1259.00000425-29
TGGTCTC150.004368995359.000004110-114
TCCTTGG501.8189894E-1259.00000435-39
Produced by FastQC (version 0.11.5)