FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n02_lib-85-4-8-2-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n02_lib-85-4-8-2-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20304
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTC390719.24251379038613No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGTGTGTTGC15737.747241922773837No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGTATCTAATCCCAG13676.732663514578407No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAAGTC11365.594956658786446No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGTATCTAATCCCAGTGTGT10885.358550039401103No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACAGGGGTATCTAATCCCAG8584.225768321513002No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCGGGGTATCTAATCCCAGTGTG4772.349290780141844No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGTATCTAATCCCAGTGTGT3881.9109535066981875No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCCCTCGATCTCCTTGGGGTATCT3711.8272261623325452No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGTC3651.7976753349093777No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTTTGGGGCACCGCCAAGTC3531.7385736800630418No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGGTATCTAATCCCAGTGTGTTG3501.723798266351458No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACAGGGGTATCTAATCCCA3141.5464933018124507No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTTGGGGTATCTAATCCCAGTGTG3041.4972419227738378No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACTGGGGTATCTAATCCCAG2711.3347123719464145No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGTATCTAATCCCAGTG2361.1623325453112687No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCGTGGGGTATCTAATCCCAGTGTG2321.1426319936958234No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGTATCTAATCCCA2241.103230890464933No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTGGGGTATCTAATCCCAGT2221.0933806146572105No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCGGGGTATCTAATCCCAG1930.9505516154452325No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATGGGGTATCTAATCC1920.9456264775413712No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGTATCTAATCCCAGTGTGTT1880.9259259259259258No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAAGTC1390.6845941686367218No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGTGGGGTATCTAATCCCAGTGT1230.6057919621749409No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTGAAGCACCGCCAGGTC1160.5713159968479117No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACTGGGGTATCTAATCCCA1120.5516154452324665No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTTGTGGGGTATCTAATCCCAG1050.5171394799054373No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATGTAAAGCACCGCCAAGTC1040.512214342001576No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTAGGGGTATCTAATCCC1000.49251379038613086No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGGGTATCTAATCCCAGTGTG940.4629629629629629No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACCGGGGTATCTAATCCCA890.43833727344365647No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACAGGGGTATCTAATCCCAGT810.39893617021276595No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGGGGGTATCTAATCCCAGTGTGT790.3890858944050433No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCAGTGGGGTATCTAATCCCAGTGT740.3644602048857368No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGTATCTAATCCCAGTGTGT710.34968479117415285No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTGGGGTATCTAATCCCAGTGTG700.34475965327029157No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCGGGGTATCTAATCCCAGTGTGT700.34475965327029157No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACACGGGGTATCTAATCCCAG660.3250591016548463No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGGTATCTAATCCCAGTGTGTT640.31520882584712373No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTTGGGGTATCTAATCCCA640.31520882584712373No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGAGTCGTATTAATGGGGTATCTA640.31520882584712373No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACTGGGGTATCTAATCCCAGT630.31028368794326244No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACTACCGGGGTATCTAATCCCAGT610.3004334121355398No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCAACGCCAAGTC580.28565799842395584No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCTCCAACAGCTGCACTTCGGAAG570.28073286052009455No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGCTCGGGGTATCTAA550.2708825847123719No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCACCAAGTC550.2708825847123719No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATTGCGGGGGTATCTAATCCCAGT520.256107171000788No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGCTCTCTACTCCCGTAGACGAGGG520.256107171000788No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGGGGTATCTAATCCCAGTGTG490.24133175728920409No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGACTACACGGGGTATCTAATCCC460.22655634357762017No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCGGGGTATCTAATCCCAGTGTGT440.21670606776989756No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATCACGTGGGGTATCTAATCCCAG400.1970055161544523No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGCTCGGGGTATCTAATCCCAG400.1970055161544523No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTGTTAGTGGGGTATCTAATCCCAG390.19208037825059102No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAAGTC390.19208037825059102No Hit
TCTCAATCTAGAACAGGCTCCTCTAGACGGGGGTATCTAATCCCAGTGTG380.18715524034672973No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTGGCAGTCAGGGGTATCTAATCC380.18715524034672973No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGACTACCCGGGGTATCTAATCCCA370.1822301024428684No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTTCGGGGTATCTAATCCCAGTGTG360.1773049645390071No Hit
TCTCAATCTAGAACAGGCTCCTCTAGTCAGGGGTATCTAATCCCAGTGTG330.16252955082742315No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAACGGGGTATCTAATCCCAGTGTG320.15760441292356187No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATGGGGTATCTAATCCCAGTGTGT300.14775413711583923No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGGGGGTATCTAATCCCAGT290.14282899921197792No Hit
TCTCAATCTAGAACAGGCTCCTCTGGACGGGGTATCTAATCCCAGTGTGT290.14282899921197792No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCTTGGGGTATCTAATCCCAG280.13790386130811663No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGACTACCGGGGTATCTAATCCCA280.13790386130811663No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCTACCCGGGGTATCTAATCCCAGT270.13297872340425532No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATTGGGGTATCTAATCCCAGTGTG270.13297872340425532No Hit
TCTCAATCTAGAACAGGCTCCTCTAGATCTCGTGGGCTCGGGGTATCTAA260.128053585500394No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACCGCCAGGTC250.12312844759653271No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGGGTATCTAATCCCAGCGTGTTGC240.1182033096926714No Hit
TCTCAATCTAGAACAGGCTCCTCTGTCTCGTGGGGTATCTAATCCCAGTG240.1182033096926714No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCGGGTC220.10835303388494878No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTGGGTCTGAGGCACCGCCAGGTC220.10835303388494878No Hit
TCTCAATCTAGAACAGGCTCCTCTAGGTCTCGTGGGGGGTATCTAATCCC220.10835303388494878No Hit
TCTCAATCTAGAACAGGCTCCTCTAGCCGGGGTATCTAATCCCAGTGTGT210.10342789598108747No Hit
TCTCAATCTAGAACAGGCTCCTCTAGAGGGATATAAAGCACTGCCAAGCC210.10342789598108747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCT20150.0293.535983
AATCTAG19900.0293.517585
ATCTAGA19950.0292.781956
CTCAATC20400.0292.107852
TCTAGAA19850.0292.02777
TCTCAAT20600.0290.70391
CAATCTA20400.0288.492654
CTAGAAC20100.0287.661688
TAGAACA21200.0273.43169
TCTGAAC150.0028291994196.666677
CAATCTG200.0066893576147.54
ATCTGAA450.00988730865.555566
CGTTTGT150.00436902659.00000465-69
GGGTCTG1200.059.00000425-29
GGGTTTG150.00436902659.00000425-29
GAGCGGT501.8189894E-1259.00000485-89
GTTTGGG150.00436902659.00000430-34
TATATTT650.059.00000495-99
GGGCACC150.00436902659.00000435-39
TTGTATT303.3120887E-759.00000495-99
Produced by FastQC (version 0.11.5)